Mouse Primed Embryonic Stem Cells Could Be Maintained and Reprogrammed on Human Amnion Epithelial Cells
文献类型：期刊论文
| 作者 | Chen, YF; Dong, ZL; Jiang, LZ; Lai, DM; Guo, LH |
| 刊名 | STEM CELLS AND DEVELOPMENT
 |
| 出版日期 | 2013 |
| 卷号 | 22期号:2页码:320-329 |
| 通讯作者 | Lai, DM (reprint author), Shanghai Jiao Tong Univ, Int Peace Matern & Child Hlth Hosp, Hengshan Rd 910, Shanghai 200030, Peoples R China.,laidongmei@hotmail.com |
| 英文摘要 | Naive and primed embryonic stem cells (ESCs) represent 2 pluripotent states of mouse embryonic stem cells (mESCs), corresponding to the pre- and postimplantation cells, respectively, in vivo. Primed ESCs are distinct from naive cells in biological characteristics, genetic features, developing potentials, and antagonistic signal pathway dependences to support undifferentiated growth. In vitro, naive mESCs are readily converted to primed cells upon transferring to primed pluripotency signaling. ESC-derived epiblast stem cells (ESD-EpiSCs) are stabilized primed cells derived from naive mESCs in vitro, and cannot be maintained with leukemia inhibitory factor (LIF) signaling with or without mouse embryonic fibroblasts as the feeder layer. Here, we show that the undifferentiated growth of ESD-EpiSCs could be maintained with the basic fibroblast growth factor employing human amnion epithelial cells (hAECs) as the feeder layer. Upon exposure to LIF, ESD-EpiSCs could undergo a reprogramming process on hAECs and be converted to naive-like cells converted ESCs (cESCs), in which naive pluripotency markers were activated, and primed markers were suppressed. DNA methylation analysis also validated the epigenetic conversion from primed to naive-like pluripotent status. The bone morphogenetic protein 4 (BMP4) is an important signaling factor in pluripotency controlling, germ cell development, and neural commitment. It showed that ESD-EpiSCs and cESCs exhibited different features toward BMP4. Our results prove that hAECs are ideal feeder cells for both naive and primed ESCs. More importantly, the primed ESCs are allowed to be reprogrammed to naive-like pluripotent cells on hAECs. These findings suggest that under suitable conditions primed ESCs have the potency of converting to naive-like ESCs. |
| 学科主题 | Cell Biology; Hematology; Research & Experimental Medicine; Transplantation |
| 类目[WOS] | Cell & Tissue Engineering ; Hematology ; Medicine, Research & Experimental ; Transplantation |
| 关键词[WOS] | SELF-RENEWAL ; PLURIPOTENCY ; DERIVATION ; EPIBLAST ; CULTURE ; DIFFERENTIATION ; INDUCTION ; MEMBRANE ; NETWORK ; PROTEIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000313577400013 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/350]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Chen, YF,Dong, ZL,Jiang, LZ,et al. Mouse Primed Embryonic Stem Cells Could Be Maintained and Reprogrammed on Human Amnion Epithelial Cells[J]. STEM CELLS AND DEVELOPMENT,2013,22(2):320-329. |
| APA | Chen, YF,Dong, ZL,Jiang, LZ,Lai, DM,&Guo, LH.(2013).Mouse Primed Embryonic Stem Cells Could Be Maintained and Reprogrammed on Human Amnion Epithelial Cells.STEM CELLS AND DEVELOPMENT,22(2),320-329. |
| MLA | Chen, YF,et al."Mouse Primed Embryonic Stem Cells Could Be Maintained and Reprogrammed on Human Amnion Epithelial Cells".STEM CELLS AND DEVELOPMENT 22.2(2013):320-329. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。