Reprogramming Fibroblasts into Bipotential Hepatic Stem Cells by Defined Factors
文献类型：期刊论文
| 作者 | Yu, B; He, ZY; You, P; Han, QW; Xiang, D; Chen, F; Wang, MJ; Liu, CC; Lin, XW; Borjigin, U |
| 刊名 | CELL STEM CELL
 |
| 出版日期 | 2013 |
| 卷号 | 13期号:3页码:328-340 |
| 通讯作者 | Wang, X (reprint author), Inner Mongolia Univ, Natl Educ Minist Mammalian Reprod Biol & Biotechn, Key Lab, Hohhot 010070, Peoples R China.,wangxin_cn@imu.edu.cn ; yphu@smmu.edu.cn |
| 英文摘要 | Recent studies have demonstrated direct reprogramming of fibroblasts into a range of somatic cell types, but to date stem or progenitor cells have only been reprogrammed for the blood and neuronal lineages. We previously reported generation of induced hepatocyte-like (iHep) cells by transduction of Gata4, Hnf1 alpha, and Foxa3 in p19 Arf null mouse embryonic fibroblasts (MEFs). Here, we show that Hnf1 beta and Foxa3, liver organogenesis transcription factors, are sufficient to reprogram MEFs into induced hepatic stem cells (iHepSCs). iHepSCs can be stably expanded in vitro and possess the potential of bidirectional differentiation into both hepatocytic and cholangiocytic lineages. In the injured liver of fumarylacetoacetate hydrolase (Fah)-deficient mice, repopulating iHepSCs become hepatocyte-like cells. They also engraft as cholangiocytes into bile ducts of mice with DDC-induced bile ductular injury. Lineage conversion into bipotential expandable iHepSCs provides a strategy to enable efficient derivation of both hepatocytes and cholangiocytes for use in disease modeling and tissue engineering. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell & Tissue Engineering ; Cell Biology |
| 关键词[WOS] | LIVER PROGENITOR CELLS ; DIRECT CONVERSION ; MOUSE FIBROBLASTS ; TRANSCRIPTION FACTORS ; DOPAMINERGIC-NEURONS ; MICE ; REGENERATION ; REPOPULATION ; HEPATOCYTES ; 3,5-DIETHOXYCARBONYL-1,4-DIHYDROCOLLIDINE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000329570800010 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/352]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Yu, B,He, ZY,You, P,et al. Reprogramming Fibroblasts into Bipotential Hepatic Stem Cells by Defined Factors[J]. CELL STEM CELL,2013,13(3):328-340. |
| APA | Yu, B.,He, ZY.,You, P.,Han, QW.,Xiang, D.,...&Hu, YP.(2013).Reprogramming Fibroblasts into Bipotential Hepatic Stem Cells by Defined Factors.CELL STEM CELL,13(3),328-340. |
| MLA | Yu, B,et al."Reprogramming Fibroblasts into Bipotential Hepatic Stem Cells by Defined Factors".CELL STEM CELL 13.3(2013):328-340. |
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