Purification and Identification of 72 kDa and 15 kDa Allergens from Broussonetia papyrifera Pollen
文献类型：期刊论文
| 作者 | Chen, Z; Zhu, N; Chen, XD; Yang, Y; Li, YP; Wu, ZL; Chen, SX |
| 刊名 | IRANIAN JOURNAL OF ALLERGY ASTHMA AND IMMUNOLOGY
 |
| 出版日期 | 2013 |
| 卷号 | 12期号:4页码:312-320 |
| 关键词 | Allergens Broussonetia papyrifera Chinese mulberry tree Pollen |
| 通讯作者 | Chen, XD (reprint author), Fudan Univ, Shanghai Huashan Hosp, 12 Urumqi Rd M, Shanghai 200040, Peoples R China.,xdchen8@hotmail.com |
| 英文摘要 | Broussonetia papyrifera (Chinese mulberry) pollen is an important source of allergens in regions surrounding Shanghai, China. To identify and purify major allergens from "B. papyrifera" pollen that reacted with serum antibodies from sensitized patients, "B. papyrifera" pollen was defatted, dried, and extracted proteins were separated using SP cationic exchange or Q anionic exchange columns. Serum samples from 29 allergic patients and 4 healthy controls were collected. Allergens in eluted fractions were identified by Western blot and enzyme-linked immunosorbent assays (ELISA) using serum samples. An inhibitory assay was used to verify allergen-specific antiserum specificity. Serum IgE of 2 patients reacted with a 15 kDa protein band. The protein was eluted with 0.1M NaCl from a SP cationic exchange column. Serum samples from the same patients positively reacted with ELISA plate coated with partially purified 15 kDa protein. Serum IgE of 11 patients reacted with a 72 kDa protein band. The protein was eluted with 0.3M NaCl from a Q anionic exchange column. Serum samples from five patients positively reacted with ELISA coated with partially purified 72 kDa protein. Our preliminary purifications identified two proteins of 72 kDa and 15 kDa as allergens derived from "B. papyrifera" pollen, which reacted with allergic patients' serum IgE. |
| 学科主题 | Allergy; Immunology |
| 类目[WOS] | Allergy ; Immunology |
| 关键词[WOS] | DESERT ENVIRONMENT ; MAJOR ALLERGEN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000323717200003 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/369]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Chen, Z,Zhu, N,Chen, XD,et al. Purification and Identification of 72 kDa and 15 kDa Allergens from Broussonetia papyrifera Pollen[J]. IRANIAN JOURNAL OF ALLERGY ASTHMA AND IMMUNOLOGY,2013,12(4):312-320. |
| APA | Chen, Z.,Zhu, N.,Chen, XD.,Yang, Y.,Li, YP.,...&Chen, SX.(2013).Purification and Identification of 72 kDa and 15 kDa Allergens from Broussonetia papyrifera Pollen.IRANIAN JOURNAL OF ALLERGY ASTHMA AND IMMUNOLOGY,12(4),312-320. |
| MLA | Chen, Z,et al."Purification and Identification of 72 kDa and 15 kDa Allergens from Broussonetia papyrifera Pollen".IRANIAN JOURNAL OF ALLERGY ASTHMA AND IMMUNOLOGY 12.4(2013):312-320. |
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