Nanog suppresses cell migration by downregulating thymosin beta 4 and Rnd3
文献类型：期刊论文
| 作者 | Zhou, YZ; Li, SS; Huang, QP; Xie, LL; Zhu, XL |
| 刊名 | JOURNAL OF MOLECULAR CELL BIOLOGY
 |
| 出版日期 | 2013 |
| 卷号 | 5期号:4页码:239-249 |
| 关键词 | differentiation migration stem cells Nanog |
| 通讯作者 | Zhu, XL (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Cell Biol, Shanghai 200031, Peoples R China.,xlzhu@sibcb.ac.cn |
| 英文摘要 | Nanog, Sox2, and Oct4 are key transcription factors critical for the pluripotency and self-renewal of embryonic stem (ES) cells. Their downregulations lead to differentiation, accompanied with changes in cell motility. Whether these factors impact cell motility directly, however, is not clear. Here we addressed this question by initially assessing their effect in non-stem cells. We found that the ectopic expression of Nanog, Sox2, or Oct4 markedly inhibited ECV304 cell migration. Detailed examinations revealed that Nanog induced disorganizations of the actin cytoskeleton and peripheral localizations of focal adhesions. These effects required its DNA-binding domain and are thus transcription dependent. Furthermore, thymosin 4 and Rnd3 were identified as its downstream targets. Their depletions in ECV304 cells by RNAi phenocopied the ectopic expression of Nanog in both cell motility and actin organization, whereas their ectopic expressions rescued the migration defect of Nanog overexpression. Both proteins were upregulated during mouse ES cell differentiation. Their levels in the pluripotent mouse P19 cells also increased upon Nanog ablation, coincident with an increase in cell motility. Moreover, persistent expression of Nanog in zebrafish embryos suppressed gastrulation and cell migration. These results indeed suggest a dual role of certain transcription factors in the orchestration of differentiation and motility. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | EMBRYONIC STEM-CELLS ; RNA INTERFERENCE ; DIFFERENTIATION ; PLURIPOTENCY ; ZEBRAFISH ; MOTILITY ; OCT4 ; SOX2 ; IDENTIFICATION ; GASTRULATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000322914000004 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/372]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhou, YZ,Li, SS,Huang, QP,et al. Nanog suppresses cell migration by downregulating thymosin beta 4 and Rnd3[J]. JOURNAL OF MOLECULAR CELL BIOLOGY,2013,5(4):239-249. |
| APA | Zhou, YZ,Li, SS,Huang, QP,Xie, LL,&Zhu, XL.(2013).Nanog suppresses cell migration by downregulating thymosin beta 4 and Rnd3.JOURNAL OF MOLECULAR CELL BIOLOGY,5(4),239-249. |
| MLA | Zhou, YZ,et al."Nanog suppresses cell migration by downregulating thymosin beta 4 and Rnd3".JOURNAL OF MOLECULAR CELL BIOLOGY 5.4(2013):239-249. |
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