Human amniotic epithelial cell feeder layers maintain iPS cell pluripotency by inhibiting endogenous DNA methyltransferase 1
文献类型：期刊论文
| 作者 | Chen, Q; Qiu, CL; Huang, YY; Jiang, LZ; Huang, Q; Guo, LH; Liu, T |
| 刊名 | EXPERIMENTAL AND THERAPEUTIC MEDICINE
 |
| 出版日期 | 2013 |
| 卷号 | 6期号:5页码:1145-1154 |
| 关键词 | human amniotic epithelial cells human induced pluripotent stem cells. DNA methyltransferase 1 pluripotency maintenance |
| 通讯作者 | Liu, T (reprint author), Shanghai Univ Tradit Chinese Med, Shanghai Geriatr Inst Chinese Med, Longhua Hosp, Room 410,558 Xiangyang Rd, Shanghai 200031, Peoples R China.,guolihe@cell-star.com.cn ; teliu79@126.com |
| 英文摘要 | Maintaining induced pluripotent stem (iPS) cells in an undifferentiated, self-renewing state during long-term cultivation is, at present, a major challenge. We previously showed that human amniotic epithelial cells (HuAECs) were able to provide a good source of feeder cells for mouse and human embryonic or spermatogonial stem cells; however, the epigenetic mechanisms have not been elucidated. In the present study, mouse embryonic fibroblasts (MEFs) and HuAECs were compared as feeder layers for the long-term culture of human iPS cells. The HuAEC feeders allowed human iPS cells to maintain a high level of alkaline phosphatase (AP) activity and to express key stem cell markers during long-term subculture whereas the MEF feeders did not,. Moreover, the HuAEC feeders significantly affected the cell cycle regulation of the iPS cells, maintaining them in the resting stage and the early stage of DNA synthesis (GO/G1 stage). Furthermore, the CpCi islands of the Nanog and Oct4 promoters were hypomethylated, while the Nanog- and Oct4-specific loci exhibited higher levels of histone H3 acetylation and lower levels of H3K 27 trimethylation in iPS cells cultured on HuAECs compared with those cultured on MEFs. The DNA methyltransferase 1 (DNMT1) expression in iPS cells cultured on HuAECs was shown to be lower than in those cultured on MEFs. In addition, DNMT1-silenced human iPS cells were able to maintain pluripotency over long-term culture on MEFs. In combination, these results suggest that endogenous DNMT1 expression in human iPS cells may be regulated by HuAEC feeder cells and that Nanog and Oct4 are crucial components required for the maintenance of iPS cells in an undifferentiated, proliferative state, capable of self-renewal. |
| 学科主题 | Research & Experimental Medicine |
| 类目[WOS] | Medicine, Research & Experimental |
| 关键词[WOS] | EMBRYONIC STEM-CELLS ; SOMATIC-CELLS ; EPIGENETIC REGULATION ; EXPRESSION ; OCT4 ; FIBROBLASTS ; GENERATION ; NANOG ; DNMT1 ; METHYLATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000324993500011 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/383]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Chen, Q,Qiu, CL,Huang, YY,et al. Human amniotic epithelial cell feeder layers maintain iPS cell pluripotency by inhibiting endogenous DNA methyltransferase 1[J]. EXPERIMENTAL AND THERAPEUTIC MEDICINE,2013,6(5):1145-1154. |
| APA | Chen, Q.,Qiu, CL.,Huang, YY.,Jiang, LZ.,Huang, Q.,...&Liu, T.(2013).Human amniotic epithelial cell feeder layers maintain iPS cell pluripotency by inhibiting endogenous DNA methyltransferase 1.EXPERIMENTAL AND THERAPEUTIC MEDICINE,6(5),1145-1154. |
| MLA | Chen, Q,et al."Human amniotic epithelial cell feeder layers maintain iPS cell pluripotency by inhibiting endogenous DNA methyltransferase 1".EXPERIMENTAL AND THERAPEUTIC MEDICINE 6.5(2013):1145-1154. |
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