SLX4 Assembles a Telomere Maintenance Toolkit by Bridging Multiple Endonucleases with Telomeres
文献类型：期刊论文
| 作者 | Wan, BB; Yin, JH; Horvath, K; Sarkar, J; Chen, Y; Wu, J; Wan, K; Lu, J; Gu, PL; Yu, EY |
| 刊名 | CELL REPORTS
 |
| 出版日期 | 2013 |
| 卷号 | 4期号:5页码:861-869 |
| 通讯作者 | Liu, Y (reprint author), NIA, Lab Mol Gerontol, NIH, 251 Bayview Dr, Baltimore, MD 21044 USA.,liuyie@mail.nih.gov ; leim@sibcb.ac.cn |
| 英文摘要 | SLX4 interacts with several endonucleases to resolve structural barriers in DNA metabolism. SLX4 also interacts with telomeric protein TRF2 in human cells. The molecular mechanism of these interactions at telomeres remains unknown. Here, we report the crystal structure of the TRF2-binding motif of SLX4 (SLX4(TBM)) in complex with the TRFH domain of TRF2 (TRF2(TRFH)) and map the interactions of SLX4 with endonucleases SLX1, XPF, and MUS81. TRF2 recognizes a unique HxLxP motif on SLX4 via the peptide-binding site in its TRFH domain. Telomeric localization of SLX4 and associated nucleases depend on the SLX4-endonuclease and SLX4-TRF2 interactions and the protein levels of SLX4 and TRF2. SLX4 assembles an endonuclease toolkit that negatively regulates telomere length via SLX1-catalyzed nucleolytic resolution of telomere DNA structures. We propose that the SLX4-TRF2 complex serves as a double-layer scaffold bridging multiple endonucleases with telomeres for recombination-based telomere maintenance. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | HOLLIDAY JUNCTION RESOLVASE ; DNA-REPAIR ; MAMMALIAN TELOMERES ; TRF1 ; LENGTH ; MECHANISM ; PROTEINS ; CIRCLES ; CELLS ; LOOP |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000324286300002 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/385]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wan, BB,Yin, JH,Horvath, K,et al. SLX4 Assembles a Telomere Maintenance Toolkit by Bridging Multiple Endonucleases with Telomeres[J]. CELL REPORTS,2013,4(5):861-869. |
| APA | Wan, BB.,Yin, JH.,Horvath, K.,Sarkar, J.,Chen, Y.,...&Lei, M.(2013).SLX4 Assembles a Telomere Maintenance Toolkit by Bridging Multiple Endonucleases with Telomeres.CELL REPORTS,4(5),861-869. |
| MLA | Wan, BB,et al."SLX4 Assembles a Telomere Maintenance Toolkit by Bridging Multiple Endonucleases with Telomeres".CELL REPORTS 4.5(2013):861-869. |
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