Tripeptidyl Peptidase II Regulates Sperm Function by Modulating Intracellular Ca2+ Stores via the Ryanodine Receptor
文献类型：期刊论文
| 作者 | Zhou, YC; Ru, YF; Wang, CM; Wang, SL; Zhou, ZM; Zhang, YL |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2013 |
| 卷号 | 8期号:6页码:e66634-e66634 |
| 通讯作者 | Zhang, YL (reprint author), Chinese Acad Sci, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, Shanghai Key Lab Mol Androl,State Key Lab Mol Bio, Shanghai, Peoples R China.,ylzhang@sibcb.ac.cn |
| 英文摘要 | Recent studies have identified Ca2+ stores in sperm cells; however, it is not clear whether these Ca2+ stores are functional and how they are mobilized. Here, in vitro and in vivo, we determined that tripeptidyl peptidase II antagonists strongly activated the cAMP/PKA signaling pathway that drives sperm capacitation-associated protein tyrosine phosphorylation. We demonstrated that in the absence of Ca2+, TPIII antagonists elevated the intracellular Ca2+ levels in sperm, resulting in a marked improvement in sperm movement, capacitation, acrosome reaction, and the in vitro fertilizing ability. This antagonist-induced release of intracellular Ca2+ could be blocked by the inhibitors of ryanodine receptors (RyRs) which are the main intracellular Ca2+ channels responsible for releasing stored Ca2+. Consistent with these results, indirect immunofluorescence assay using anti-RyR antibodies further validated the presence of RyR(3) in the acrosomal region of mature sperm. Thus, TPPII can regulate sperm maturation by modulating intracellular Ca2+ stores via the type 3 RyR. |
| 学科主题 | Science & Technology - Other Topics |
| 类目[WOS] | Multidisciplinary Sciences |
| 关键词[WOS] | PROTEIN-TYROSINE PHOSPHORYLATION ; ACROSOME REACTION ; MOUSE SPERMATOZOA ; CAPACITATION ; PROTEASOME ; CALCIUM ; FERTILIZATION ; PROGESTERONE ; INHIBITION ; PURIFICATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000322342800088 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/395]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhou, YC,Ru, YF,Wang, CM,et al. Tripeptidyl Peptidase II Regulates Sperm Function by Modulating Intracellular Ca2+ Stores via the Ryanodine Receptor[J]. PLOS ONE,2013,8(6):e66634-e66634. |
| APA | Zhou, YC,Ru, YF,Wang, CM,Wang, SL,Zhou, ZM,&Zhang, YL.(2013).Tripeptidyl Peptidase II Regulates Sperm Function by Modulating Intracellular Ca2+ Stores via the Ryanodine Receptor.PLOS ONE,8(6),e66634-e66634. |
| MLA | Zhou, YC,et al."Tripeptidyl Peptidase II Regulates Sperm Function by Modulating Intracellular Ca2+ Stores via the Ryanodine Receptor".PLOS ONE 8.6(2013):e66634-e66634. |
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