Characterization of the intramolecular G-quadruplex promoting activity of Est1
文献类型：期刊论文
| 作者 | Li, QJ; Tong, XJ; Duan, YM; Zhou, JQ |
| 刊名 | FEBS LETTERS
 |
| 出版日期 | 2013 |
| 卷号 | 587期号:6页码:659-665 |
| 关键词 | Est1 G-quadruplex Telomerase Telomeres |
| 通讯作者 | Zhou, JQ (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai, Peoples R China.,jqzhou@sibcb.ac.cn |
| 英文摘要 | In the budding yeast Saccharomyces cerevisiae, telomeric DNA includes TG(1-3)/C(1-3)A double-stranded DNA and a protruding G-rich overhang. Our previous studies revealed that the telomerase regulatory subunit Est1 promotes telomeric single-stranded DNA to form intermolecular G-quadruplex in vitro, and this activity is required for telomere replication and protection in vivo. In this study, we further characterized the G-quadruplex promoting activity of Est1. Here we report that Est1 is able to promote the single-stranded oligonucleotide of (TGTGTGGG)(4), which mimics the natural telomeric DNA, to form intramolecular G-quadruplex. Therefore, it remains possible that the intramolecular G-quadruplex promoting activity of Est1 is biologically relevant in telomere replication in vivo. (C) 2013 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics; Cell Biology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics ; Cell Biology |
| 关键词[WOS] | TELOMERE-BINDING-PROTEIN ; SACCHAROMYCES-CEREVISIAE ; YEAST TELOMERASE ; CATALYTIC SUBUNIT ; DNA STRUCTURES ; IN-VIVO ; SEQUENCE ; REPLICATION ; SENESCENCE ; ELONGATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000315947400019 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/409]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Li, QJ,Tong, XJ,Duan, YM,et al. Characterization of the intramolecular G-quadruplex promoting activity of Est1[J]. FEBS LETTERS,2013,587(6):659-665. |
| APA | Li, QJ,Tong, XJ,Duan, YM,&Zhou, JQ.(2013).Characterization of the intramolecular G-quadruplex promoting activity of Est1.FEBS LETTERS,587(6),659-665. |
| MLA | Li, QJ,et al."Characterization of the intramolecular G-quadruplex promoting activity of Est1".FEBS LETTERS 587.6(2013):659-665. |
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