NuRD Blocks Reprogramming of Mouse Somatic Cells into Pluripotent Stem Cells
文献类型：期刊论文
| 作者 | Luo, M; Ling, T; Xie, WB; Sun, H; Zhou, YG; Zhu, QY; Shen, ML; Zong, L; Lyu, GL; Zhao, Y |
| 刊名 | STEM CELLS
 |
| 出版日期 | 2013 |
| 卷号 | 31期号:7页码:1278-1286 |
| 关键词 | Mbd3 NuRD Induced pluripotent stem cells Epigenetic regulation Nanog Reprogramming efficiency |
| 通讯作者 | Grummt, I (reprint author), DKFZ ZMBH Alliance, German Canc Res Ctr, INF 581, D-69120 Heidelberg, Germany.,weitao@pku.edu.cn ; luzg@pkusz.edu.cn ; i.grummt@dkfz.de |
| 英文摘要 | Reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) by overexpression of a defined set of transcription factors requires epigenetic changes in pluripotency genes. Nuclear reprogramming is an inefficient process and the molecular mechanisms that reset the epigenetic state during iPSC generation are largely unknown. Here, we show that downregulation of the nucleosome remodeling and deacetylation (NuRD) complex is required for efficient reprogramming. Overexpression of Mbd3, a subunit of NuRD, inhibits induction of iPSCs by establishing heterochromatic features and silencing embryonic stem cell-specific marker genes, including Oct4 and Nanog. Depletion of Mbd3, on the other hand, improves reprogramming efficiency and facilitates the formation of pluripotent stem cells that are capable of generating viable chimeric mice, even in the absence of c-Myc or Sox2. The results establish Mbd3/NuRD as an important epigenetic regulator that restricts the expression of key pluripotency genes, suggesting that drug-induced downregulation of Mbd3/NuRD may be a powerful means to improve the efficiency and fidelity of reprogramming. STEM Cells2013;31:1278-1286 |
| 学科主题 | Cell Biology; Biotechnology & Applied Microbiology; Oncology; Hematology |
| 类目[WOS] | Cell & Tissue Engineering ; Biotechnology & Applied Microbiology ; Oncology ; Cell Biology ; Hematology |
| 关键词[WOS] | SMALL-MOLECULE COMPOUNDS ; HISTONE DEACETYLASE ; HUMAN FIBROBLASTS ; DEFINED FACTORS ; COMPLEX ; INDUCTION ; NANOG ; COMPONENT ; EXPRESSION ; RECRUITMENT |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000321339000007 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/411]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Luo, M,Ling, T,Xie, WB,et al. NuRD Blocks Reprogramming of Mouse Somatic Cells into Pluripotent Stem Cells[J]. STEM CELLS,2013,31(7):1278-1286. |
| APA | Luo, M.,Ling, T.,Xie, WB.,Sun, H.,Zhou, YG.,...&Grummt, I.(2013).NuRD Blocks Reprogramming of Mouse Somatic Cells into Pluripotent Stem Cells.STEM CELLS,31(7),1278-1286. |
| MLA | Luo, M,et al."NuRD Blocks Reprogramming of Mouse Somatic Cells into Pluripotent Stem Cells".STEM CELLS 31.7(2013):1278-1286. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。