Depletion of PHF14, a novel histone-binding protein gene, causes neonatal lethality in mice due to respiratory failure
文献类型：期刊论文
| 作者 | Huang, Q; Zhang, L; Wang, YG; Zhang, CY; Zhou, SH; Yang, G; Li, ZQ; Gao, X; Chen, ZJ; Zhang, Z |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2013 |
| 卷号 | 45期号:8页码:622-633 |
| 关键词 | plant homeodomain (PHD) finger PHF14 epigenetic knockout mice |
| 通讯作者 | Chen, ZJ (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,zjchen@sibcb.ac.cn ; zhangzhe@sibcb.ac.cn |
| 英文摘要 | The plant homeodomain (PHD) finger is identified in many chromatin-binding proteins, and functions as a reader that recognizes specific epigenetic marks on histone tails, bridging transcription factors and their associated complexes to chromatin, and regulating gene expression. PHD finger-containing proteins perform many biological functions and are involved in many human diseases including cancer. PHF14 is predicted to code for a protein with multiple PHD fingers. However, its function is unidentified. The aim of this study is to characterize PHF14 and investigate its biological significance by employing multiple approaches including mouse gene-targeting knockout, and molecular cloning and characterization. Three transcripts of PHF14 in human cell lines were identified by reverse transcriptase polymerase chain reaction. Two isoforms of PHF14 (PHF14 and PHF14) were cloned in this study. It was found that PHF14 was ubiquitously expressed in mouse tissues and human cell lines. PHF14, the major isoform of PHF14, was localized in the nucleus and also bound to chromatin during cell division. Interestingly, co-immunoprecipitation results suggested that PHF14 bound to histones via its PHD fingers. Strikingly, gene-targeting knockout of PHF14 in mice resulted in a neonatal lethality due to respiratory failure. Pathological analysis revealed severe disorders of tissue and cell structures in multiple organs, particularly in the lungs. These results indicated that PHF14 might be an epigenetic regulator and play an important role in the development of multiple organs in mouse. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | PHD FINGER ; EPIGENETIC MARKS ; GROWTH-FACTOR ; CHROMATIN ; MUTATIONS ; DOMAIN ; METHYLATION ; REGULATOR ; COMPLEXES ; JADE-1 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000322334000002 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/419]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Huang, Q,Zhang, L,Wang, YG,et al. Depletion of PHF14, a novel histone-binding protein gene, causes neonatal lethality in mice due to respiratory failure[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2013,45(8):622-633. |
| APA | Huang, Q.,Zhang, L.,Wang, YG.,Zhang, CY.,Zhou, SH.,...&Zhang, Z.(2013).Depletion of PHF14, a novel histone-binding protein gene, causes neonatal lethality in mice due to respiratory failure.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,45(8),622-633. |
| MLA | Huang, Q,et al."Depletion of PHF14, a novel histone-binding protein gene, causes neonatal lethality in mice due to respiratory failure".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 45.8(2013):622-633. |
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