Established Thymic Epithelial Progenitor/Stem Cell-Like Cell Lines Differentiate into Mature Thymic Epithelial Cells and Support T Cell Development
文献类型：期刊论文
| 作者 | Chen, PF; Zhang, J; Zhan, Y; Su, JJ; Du, YR; Xu, GL; Shi, YF; Siebenlist, U; Zhang, XR |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2013 |
| 卷号 | 8期号:9页码:42006 |
| 通讯作者 | Zhang, XR (reprint author), Chinese Acad Sci, Key Lab Stem Cell Biol, Inst Hlth Sci, Shanghai, Peoples R China.,xrzhang@sibs.ac.cn |
| 英文摘要 | Common thymic epithelial progenitor/stem cells (TEPCs) differentiate into cortical and medullary thymic epithelial cells (TECs), which are required for the development and selection of thymocytes. Mature TEC lines have been widely established. However, the establishment of TEPC lines is rarely reported. Here we describe the establishment of thymic epithelial stomal cell lines, named TSCs, from fetal thymus. TSCs express some of the markers present on tissue progenitor/stem cells such as Sca-1. Gene expression profiling verifies the thymic identity of TSCs. RANK stimulation of these cells induces expression of autoimmune regulator (Aire) and Aire-dependent tissue-restricted antigens (TRAs) in TSCs in vitro. TSCs could be differentiated into medullary thymic epithelial cell-like cells with exogenously expressed NF-kappa B subunits ReIB and p52. Importantly, upon transplantation under the kidney capsules of nude mice, TSCs are able to differentiate into mature TEC-like cells that can support some limited development of T cells in vivo. These findings suggest that the TSC lines we established bear some characteristics of TEPC cells and are able to differentiate into functional TEC-like cells in vitro and in vivo. The cloned TEPC-like cell lines may provide useful tools to study the differentiation of mature TEC cells from precursors. |
| 学科主题 | Science & Technology - Other Topics |
| 类目[WOS] | Multidisciplinary Sciences |
| 关键词[WOS] | EMBRYONIC STEM-CELLS ; AUTOIMMUNE REGULATOR ; NEGATIVE SELECTION ; SELF-TOLERANCE ; AIRE ; MICROENVIRONMENT ; THYMOCYTES ; IDENTIFICATION ; PROLIFERATION ; MAINTENANCE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000326520200090 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/424]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Chen, PF,Zhang, J,Zhan, Y,et al. Established Thymic Epithelial Progenitor/Stem Cell-Like Cell Lines Differentiate into Mature Thymic Epithelial Cells and Support T Cell Development[J]. PLOS ONE,2013,8(9):42006. |
| APA | Chen, PF.,Zhang, J.,Zhan, Y.,Su, JJ.,Du, YR.,...&Zhang, XR.(2013).Established Thymic Epithelial Progenitor/Stem Cell-Like Cell Lines Differentiate into Mature Thymic Epithelial Cells and Support T Cell Development.PLOS ONE,8(9),42006. |
| MLA | Chen, PF,et al."Established Thymic Epithelial Progenitor/Stem Cell-Like Cell Lines Differentiate into Mature Thymic Epithelial Cells and Support T Cell Development".PLOS ONE 8.9(2013):42006. |
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