The microtubule-associated protein DCAMKL1 regulates osteoblast function via repression of Runx2
文献类型：期刊论文
| 作者 | Zou, WG; Greenblatt, MB; Brady, N; Lotinun, S; Zhai, B; de Rivera, H; Singh, A; Sun, J; Gygi, SP; Baron, R |
| 刊名 | JOURNAL OF EXPERIMENTAL MEDICINE
 |
| 出版日期 | 2013 |
| 卷号 | 210期号:9页码:1793-1806 |
| 通讯作者 | Zou, WG (reprint author), Chinese Acad Sci, State Key Lab Cell Biol, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China.,zouwg94@sibcb.ac.cn |
| 英文摘要 | Osteoblasts are responsible for the formation and mineralization of the skeleton. To identify novel regulators of osteoblast differentiation, we conducted an unbiased forward genetic screen using a lentiviral-based shRNA library. This functional genomics analysis led to the identification of the microtubule-associated protein DCAMKL1 (Doublecortin-like and CAM kinase-like 1) as a novel regulator of osteogenesis. Mice with a targeted disruption of Dcamkl1 displayed elevated bone mass secondary to increased bone formation by osteoblasts. Molecular experiments demonstrated that DCAMKL1 represses osteoblast activation by antagonizing Runx2, the master transcription factor in osteoblasts. Key elements of the cleidocranial dysplasia phenotype observed in Runx2(+/-) mice are reversed by the introduction of a Dcamkl1-null allele. Our results establish a genetic linkage between these two proteins in vivo and demonstrate that DCAMKL1 is a physiologically relevant regulator of anabolic bone formation. |
| 学科主题 | Immunology; Research & Experimental Medicine |
| 类目[WOS] | Immunology ; Medicine, Research & Experimental |
| 关键词[WOS] | DOUBLECORTIN-LIKE-KINASE ; CLEIDOCRANIAL DYSPLASIA ; SERINE PHOSPHORYLATION ; TRANSCRIPTION FACTOR ; SKELETAL DEVELOPMENT ; NEURONAL MIGRATION ; BONE-FORMATION ; STEM-CELL ; MASS ; DIFFERENTIATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000323644800013 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/430]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zou, WG,Greenblatt, MB,Brady, N,et al. The microtubule-associated protein DCAMKL1 regulates osteoblast function via repression of Runx2[J]. JOURNAL OF EXPERIMENTAL MEDICINE,2013,210(9):1793-1806. |
| APA | Zou, WG.,Greenblatt, MB.,Brady, N.,Lotinun, S.,Zhai, B.,...&Jones, DC.(2013).The microtubule-associated protein DCAMKL1 regulates osteoblast function via repression of Runx2.JOURNAL OF EXPERIMENTAL MEDICINE,210(9),1793-1806. |
| MLA | Zou, WG,et al."The microtubule-associated protein DCAMKL1 regulates osteoblast function via repression of Runx2".JOURNAL OF EXPERIMENTAL MEDICINE 210.9(2013):1793-1806. |
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