Leucine-specific domain modulates the aminoacylation and proofreading functional cycle of bacterial leucyl-tRNA synthetase
文献类型：期刊论文
| 作者 | Yan, W; Tan, M; Eriani, G; Wang, ED |
| 刊名 | NUCLEIC ACIDS RESEARCH
 |
| 出版日期 | 2013 |
| 卷号 | 41期号:9页码:4988-4998 |
| 通讯作者 | Wang, ED (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol,Ctr RNA Res, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,edwang@sibcb.ac.cn |
| 英文摘要 | The leucine-specific domain (LSD) is a compact well-ordered module that participates in positioning of the conserved KMSKS catalytic loop in most leucyl-tRNA synthetases (LeuRSs). However, the LeuRS from Mycoplasma mobile (MmLeuRS) has a tetrapeptide GKDG instead of the LSD. Here, we show that the tetrapeptide GKDG can confer tRNA charging and post-transfer editing activity when transplanted into an inactive Escherichia coli LeuRS (EcLeuRS) that has had its LSD deleted. Reciprocally, the LSD, together with the CP1-editing domain of EcLeuRS, can cooperate when inserted into the scaffold of the minimal MmLeuRS, and this generates an enzyme nearly as active as EcLeuRS. Further, we show that LSD participates in tRNA(Leu) recognition and favours the binding of tRNAs harbouring a large loop in the variable arm. Additional analysis established that the Lys598 in the LSD is the critical residue for tRNA binding. Conversion of Lys598 to Ala simultaneously reduces the tRNA-binding strength and aminoacylation and editing capacities, indicating that these factors are subtly connected and controlled at the level of the LSD. The present work provides a novel framework of co-evolution between LeuRS and its cognate tRNA through LSD. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | ESCHERICHIA-COLI TRNA(LEU) ; CRYSTAL-STRUCTURE ; VARIABLE POCKET ; EDITING-DOMAIN ; ACCEPTOR IDENTITY ; QUALITY-CONTROL ; NUCLEOTIDES ; RECOGNITION ; SEQUENCE ; COMPLEX |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000318570000028 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/431]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Yan, W,Tan, M,Eriani, G,et al. Leucine-specific domain modulates the aminoacylation and proofreading functional cycle of bacterial leucyl-tRNA synthetase[J]. NUCLEIC ACIDS RESEARCH,2013,41(9):4988-4998. |
| APA | Yan, W,Tan, M,Eriani, G,&Wang, ED.(2013).Leucine-specific domain modulates the aminoacylation and proofreading functional cycle of bacterial leucyl-tRNA synthetase.NUCLEIC ACIDS RESEARCH,41(9),4988-4998. |
| MLA | Yan, W,et al."Leucine-specific domain modulates the aminoacylation and proofreading functional cycle of bacterial leucyl-tRNA synthetase".NUCLEIC ACIDS RESEARCH 41.9(2013):4988-4998. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。