Characterization of the GufA subfamily member SLC39A11/Zip11 as a zinc transporter
文献类型：期刊论文
| 作者 | Yu, Y; Wu, AM; Zhang, ZZ; Yan, G; Zhang, F; Zhang, LH; Shen, XY; Hu, RG; Zhang, Y; Zhang, KY |
| 刊名 | JOURNAL OF NUTRITIONAL BIOCHEMISTRY
 |
| 出版日期 | 2013 |
| 卷号 | 24期号:10页码:1697-1708 |
| 关键词 | Zinc transporter SLC39A11/ZIP11 ZIP family MRE MTF-1 Zinc homeostasis |
| 通讯作者 | Wang, FD (reprint author), Zhejiang Univ, Sch Publ Hlth, Inst Nutr & Food Safety, Dept Nutr, Hangzhou 310058, Zhejiang, Peoples R China.,yanzhang01@sibs.ac.cn ; zkeying@yahoo.com ; fwang@zju.edu.cn |
| 英文摘要 | Cellular zinc influx and efflux are maintained by two major transporter families, the ZIP (SLC39A) and ZnT (SLC30A or CDF) molecules. The functions of one molecule in this class, ZIP11/SLC39A11, remain unclear. Bioinformatics analysis of the distribution and evolutionary relationships of different ZIP members in eukaryotes and prokaryotes indicated that Zip11, the sole member of gufA subfamily, is an ancient ZIP family member that might have originated in early eukaryotic ancestors. Murine Zip11 mRNA is abundantly expressed in testes and the digestive system including stomach, ileum and cecum. Analysis of cellular zinc content, metallothionein levels, and cell viability under high or low zinc conditions in cells transfected with a murine Zip11 expression plasmid, suggest that Zip11 is a zinc importer. Further, cellular zinc concentrations and metallothionein levels decreased when Zip11 was knocked down. In mice supplemented with zinc, both mRNA and protein levels of Zip11 were slightly up-regulated in several tissues. The metal response element sequences (MREs) upstream of the first exon of Zip11 responded to elevated extracellular zinc concentrations, as assessed by luciferase reporter assays. Mutagenic analysis showed that several of the MREs could regulate Zip11 promoter activity, and metal-responsive transcription factor-1 (MTF-1) was shown to be involved in this process. Collectively, these data suggest that Zip11 has unique protein sequence and structure features, it functions as a cellular zinc transporter, and its expression is at least partially regulated by zinc via hMTF-1 binding to MREs of the Zip11 promoter. (c) 2013 Elsevier Inc. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Nutrition & Dietetics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Nutrition & Dietetics |
| 关键词[WOS] | TRANSCRIPTION FACTOR MTF-1 ; DIETARY ZINC ; ACRODERMATITIS-ENTEROPATHICA ; IN-VIVO ; MOUSE ; CELLS ; EXPRESSION ; GENE ; ZIP4 ; PROTEIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000325123000004 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/435]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Yu, Y,Wu, AM,Zhang, ZZ,et al. Characterization of the GufA subfamily member SLC39A11/Zip11 as a zinc transporter[J]. JOURNAL OF NUTRITIONAL BIOCHEMISTRY,2013,24(10):1697-1708. |
| APA | Yu, Y.,Wu, AM.,Zhang, ZZ.,Yan, G.,Zhang, F.,...&Wang, FD.(2013).Characterization of the GufA subfamily member SLC39A11/Zip11 as a zinc transporter.JOURNAL OF NUTRITIONAL BIOCHEMISTRY,24(10),1697-1708. |
| MLA | Yu, Y,et al."Characterization of the GufA subfamily member SLC39A11/Zip11 as a zinc transporter".JOURNAL OF NUTRITIONAL BIOCHEMISTRY 24.10(2013):1697-1708. |
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