Different Effects of Androgen on the Expression of Fut1, Fut2, Fut4 and Fut9 in Male Mouse Reproductive Tract
文献类型：期刊论文
| 作者 | Wang, CM; Hu, SG; Ru, YF; Yao, GX; Ma, WB; Gu, YH; Chu, C; Wang, SL; Zhou, ZM; Liu, Q |
| 刊名 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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| 出版日期 | 2013 |
| 卷号 | 14期号:11页码:23188-23202 |
| 关键词 | fucosyltransferase androgen male reproductive tract |
| 通讯作者 | Zhou, YC (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai Key Lab Mol Androl,State Key Lab Mol Bio, Shanghai 200031, Peoples R China.,cmwang@sibcb.ac.cn ; sghu@sibs.ac.cn ; ruyanfei@sibcb.ac.cn ; ygx850226@163.com ; mawubin@sibcb.ac.cn ; yhgu@sibs.ac.cn ; chuchen@sibcb.ac.cn ; wangshl@njmu.edu.cn ; zhouzm@njmu.edu.cn ; qliu@sibs.ac.cn ; zhouych@sibcb.ac.cn ; ylzhang@sibcb.ac.cn |
| 英文摘要 | The -(1,2) fucosyltransferases (Fut1 and Fut2) and -(1,3) fucosyltransferases (Fut4, Fut9) are responsible for the synthesis of Lewis X (LeX) and Lewis Y (LeY) conjugated to glycoproteins. We recently reported that these fucosyltransferases were differentially expressed in the reproductive tract of male mouse. Here, we studied the effect of androgen on fucosyltransferase expression through the use of mouse castration models. We found that Fut1 mRNA and Fut4 mRNA were upregulated, while Fut2 mRNA and Fut9 mRNA were downregulated by androgen in the caput epididymis. However, in the vas deferens and prostate, only Fut4 mRNA and Fut2 mRNA were respectively upregulated following exposure to androgen. In the seminal vesicle, all fucosyltransferases, with the exception of Fut9, were upregulated. We identified the androgen receptor binding sites (ARBSs) of Fut2, Fut4 and Fut9 in the caput epididymis. Luciferase assay for these ARBSs is able to provide an indication as to why Fut4 and Fut9 are differently expressed and regulated by androgen, although they catalyze the same -(1,3) fucose linkage. Our study showed that androgen could differentially regulate the expression of these fucosyltransferases and provided an insight into the characteristic distribution of each fucosyltransferase responsible for LeX/LeY biosynthesis in the male reproductive tract. |
| 学科主题 | Biochemistry & Molecular Biology; Chemistry |
| 类目[WOS] | Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary |
| 关键词[WOS] | LEWIS-Y ANTIGENS ; MESSENGER-RNA ; CELL-SURFACE ; EPIDIDYMIS ; APOPTOSIS ; BIOSYNTHESIS ; MICE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000328624400115 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/441]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wang, CM,Hu, SG,Ru, YF,et al. Different Effects of Androgen on the Expression of Fut1, Fut2, Fut4 and Fut9 in Male Mouse Reproductive Tract[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2013,14(11):23188-23202. |
| APA | Wang, CM.,Hu, SG.,Ru, YF.,Yao, GX.,Ma, WB.,...&Zhang, YL.(2013).Different Effects of Androgen on the Expression of Fut1, Fut2, Fut4 and Fut9 in Male Mouse Reproductive Tract.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,14(11),23188-23202. |
| MLA | Wang, CM,et al."Different Effects of Androgen on the Expression of Fut1, Fut2, Fut4 and Fut9 in Male Mouse Reproductive Tract".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 14.11(2013):23188-23202. |
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