Synaptic acetylcholinesterase targeted by microRNA-212 functions as a tumor suppressor in non-small cell lung cancer
文献类型：期刊论文
| 作者 | Lu, L; Zhang, XJ; Zhang, B; Wu, J; Zhang, XJ |
| 刊名 | INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
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| 出版日期 | 2013 |
| 卷号 | 45期号:11页码:2530-2540 |
| 关键词 | Acetylcholinesterase Non-small cell lung cancer MiR-212 Apoptosis Tumor suppressor |
| 通讯作者 | Zhang, XJ (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Cell Biol, Shanghai 200031, Peoples R China.,xjzhang@sibcb.ac.cn |
| 英文摘要 | Acetylcholinesterase expression is modulated in various types of tumor, which suggests it is associated with tumor development; however, the mechanism of acetylcholinesterase gene regulation in tumors remains unclear. Here, we report that acetylcholinesterase is aberrantly expressed in non-small cell lung cancer and is an evolutionarily conserved functional target of miR-212. Acetylcholinesterase expression was negatively regulated by miR-212 in vitro and was inversely correlated with miR-212 expression in vivo. In addition, acetylcholinesterase levels were increased, and miR-212 levels decreased, in non-small cell lung cancer cells during cisplatin-induced apoptosis. We further determined that acetylcholinesterase acted as a pro-apoptotic gene in non-small cell lung cells; and attenuated the growth of xenografts in nude mice when upregulated. In contrast, elevated miR-212 levels preserved the protective effect of acetylcholinesterase silencing by RNA interference against cisplatin-induced apoptosis, whereas restoration of miR-212-resistant synaptic acetylcholinesterase expression inhibited the miR-212 anti-apoptotic function. The results demonstrated that miR-212 exerted an anti-apoptotic effect through direct repression of synaptic acetylcholinesterase expression in non-small cell lung cancer cells. Taken together, our study revealed that synaptic acetylcholinesterase may be a tumor suppressor and is modulated by miR-212 in non-small cell lung cancer. (C) 2013 Elsevier Ltd. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Cell Biology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Cell Biology |
| 关键词[WOS] | CHOLINESTERASE ACTIVITY ; CISPLATIN RESISTANCE ; BREAST-CANCER ; EXPRESSION ; APOPTOSIS ; CARCINOMA ; DIAGNOSIS ; GENES ; INDUCTION ; DELETION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000327914800019 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/442]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Lu, L,Zhang, XJ,Zhang, B,et al. Synaptic acetylcholinesterase targeted by microRNA-212 functions as a tumor suppressor in non-small cell lung cancer[J]. INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY,2013,45(11):2530-2540. |
| APA | Lu, L,Zhang, XJ,Zhang, B,Wu, J,&Zhang, XJ.(2013).Synaptic acetylcholinesterase targeted by microRNA-212 functions as a tumor suppressor in non-small cell lung cancer.INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY,45(11),2530-2540. |
| MLA | Lu, L,et al."Synaptic acetylcholinesterase targeted by microRNA-212 functions as a tumor suppressor in non-small cell lung cancer".INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY 45.11(2013):2530-2540. |
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