Novel Recombinant Hepatitis B Virus Vectors Efficiently Deliver Protein and RNA Encoding Genes into Primary Hepatocytes
文献类型：期刊论文
| 作者 | Hong, R; Bai, WY; Zhai, JW; Liu, W; Li, XY; Zhang, JM; Cui, XX; Zhao, X; Ye, XL; Deng, Q |
| 刊名 | JOURNAL OF VIROLOGY
 |
| 出版日期 | 2013 |
| 卷号 | 87期号:12页码:6615-6624 |
| 通讯作者 | Liu, J (reprint author), Fudan Univ, Key Lab Med Mol Virol, Minist Hlth, Shanghai 200433, Peoples R China.,liujing212@fudan.edu.cn ; yhxie@fudan.edu.cn |
| 英文摘要 | Hepatitis B virus (HBV) has extremely restricted host and hepatocyte tropism. HBV-based vectors could form the basis of novel therapies for chronic hepatitis B and other liver diseases and would also be invaluable for the study of HBV infection. Previous attempts at developing HBV-based vectors encountered low yields of recombinant viruses and/or lack of sufficient infectivity/cargo gene expression in primary hepatocytes, which hampered follow-up applications. In this work, we constructed a novel vector based on a naturally occurring, highly replicative HBV mutant with a 207-bp deletion in the preS1/polymerase spacer region. By applying a novel insertion strategy that preserves the continuity of the polymerase open reading frame (ORF), recombinant HBV (rHBV) carrying protein or small interfering RNA (siRNA) genes were obtained that replicated and were packaged efficiently in cultured hepatocytes. We demonstrated that rHBV expressing a fluorescent reporter (DsRed) is highly infective in primary tree shrew hepatocytes, and rHBV expressing HBV-targeting siRNA successfully inhibited antigen expression from coinfected wild-type HBV. This novel HBV vector will be a powerful tool for hepatocyte-targeting gene delivery, as well as the study of HBV infection. |
| 学科主题 | Virology |
| 类目[WOS] | Virology |
| 关键词[WOS] | CHRONIC HBV INFECTION ; IN-VITRO ; REVERSE-TRANSCRIPTASE ; EXPRESSION ; CELLS ; REPLICATION ; DOMAINS ; BINDING ; GENOME ; LENGTH |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000319508600009 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/451]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Hong, R,Bai, WY,Zhai, JW,et al. Novel Recombinant Hepatitis B Virus Vectors Efficiently Deliver Protein and RNA Encoding Genes into Primary Hepatocytes[J]. JOURNAL OF VIROLOGY,2013,87(12):6615-6624. |
| APA | Hong, R.,Bai, WY.,Zhai, JW.,Liu, W.,Li, XY.,...&Xie, YH.(2013).Novel Recombinant Hepatitis B Virus Vectors Efficiently Deliver Protein and RNA Encoding Genes into Primary Hepatocytes.JOURNAL OF VIROLOGY,87(12),6615-6624. |
| MLA | Hong, R,et al."Novel Recombinant Hepatitis B Virus Vectors Efficiently Deliver Protein and RNA Encoding Genes into Primary Hepatocytes".JOURNAL OF VIROLOGY 87.12(2013):6615-6624. |
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