Spink13, an Epididymis-specific Gene of the Kazal-type Serine Protease Inhibitor (SPINK) Family, Is Essential for the Acrosomal Integrity and Male Fertility
文献类型：期刊论文
| 作者 | Ma, L; Yu, HG; Ni, ZM; Hu, SG; Ma, WB; Chu, C; Liu, Q; Zhang, YL; Ma, L; Yu, HG |
| 刊名 | JOURNAL OF BIOLOGICAL CHEMISTRY
 |
| 出版日期 | 2013 |
| 卷号 | 288期号:14页码:10154-10165 |
| 通讯作者 | Liu, Q (reprint author), Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,qliu@sibcb.ac.cn |
| 英文摘要 | Sperm maturation involves numerous surface modifications by a variety of secreted proteins from epididymal epithelia. The sperm surface architecture depends on correct localization of its components and highlights the importance of the sequence of the proteolytic processing of the sperm surface in the epididymal duct. The presence of several protease inhibitors from different families is consistent with the hypothesis that correctly timed epididymal protein processing is essential for proper sperm maturation. Here we show that the rat (Rattus norvegicus) epididymis-specific gene Spink13, an androgen-responsive serine protease inhibitor, could bind to the sperm acrosome region. Furthermore, knockdown of Spink13 in vivo dramatically enhanced the acrosomal exocytosis during the process of capacitation and thus led to a significant reduction in male fertility, indicating that Spink13 was essential for sperm maturation. We conclude that blockade of SPINK13 may provide a new putative target for post-testicular male contraceptives. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | ANGIOTENSIN-CONVERTING ENZYME ; IN-VITRO FERTILIZATION ; AMINO-ACID-SEQUENCE ; TRYPSIN-INHIBITOR ; SPERM MATURATION ; MOUSE SPERMATOZOA ; ANCHORED PROTEIN ; CHRONIC-PANCREATITIS ; NETHERTON-SYNDROME ; MOLECULAR-CLONING |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000317114000056 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/453]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Ma, L,Yu, HG,Ni, ZM,et al. Spink13, an Epididymis-specific Gene of the Kazal-type Serine Protease Inhibitor (SPINK) Family, Is Essential for the Acrosomal Integrity and Male Fertility[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2013,288(14):10154-10165. |
| APA | Ma, L.,Yu, HG.,Ni, ZM.,Hu, SG.,Ma, WB.,...&Zhang, YL.(2013).Spink13, an Epididymis-specific Gene of the Kazal-type Serine Protease Inhibitor (SPINK) Family, Is Essential for the Acrosomal Integrity and Male Fertility.JOURNAL OF BIOLOGICAL CHEMISTRY,288(14),10154-10165. |
| MLA | Ma, L,et al."Spink13, an Epididymis-specific Gene of the Kazal-type Serine Protease Inhibitor (SPINK) Family, Is Essential for the Acrosomal Integrity and Male Fertility".JOURNAL OF BIOLOGICAL CHEMISTRY 288.14(2013):10154-10165. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。