Structural basis for recognition of the third SH3 domain of full-length R85 (R85FL)/ponsin by ataxin-7
文献类型：期刊论文
| 作者 | Jiang, YJ; Zhou, CJ; Zhou, ZR; Wu, M; Hu, HY |
| 刊名 | FEBS LETTERS
 |
| 出版日期 | 2013 |
| 卷号 | 587期号:18页码:2905-2911 |
| 关键词 | Ataxin-7 Proline-rich region R85FL/ponsin SH3 domain Polyglutamine Inclusion body |
| 通讯作者 | Hu, HY (reprint author), Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,hyhu@sibcb.ac.cn |
| 英文摘要 | Ataxin-7 (Atx7) is a component of the nuclear transcription co-activator complex; its polyglutamine (polyQ) expansion may cause nuclear accumulation and recruit numerous proteins to the intranuclear inclusion bodies. Full-length R85 (R85FL) is such a protein sequestered by polyQ-expanded Atx7. Here, we report that Atx7 specifically interacts with the third SH3 domain (SH3C) of R85FL through its second portion of proline-rich region (PRR). NMR structural analysis of the SH3C domain and its complex with PRR revealed that SH3C contains a large negatively charged surface for binding with the RRTR motif of Atx7. Microscopy imaging demonstrated that sequestration of R85FL by the polyQ-expanded Atx7 in cell is mediated by this specific SH3C-PRR interaction, which is implicated in the pathogenesis of spinocerebellar ataxia 7. Structured summary of protein interactions: Atx7 PP2 and SH3C bind by isothermal titration calorimetry (View Interaction: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12) Atx7 binds to SH3C by pull down (View interaction) Atx7(100Q) and SH3C colocalize by fluorescence microscopy (View interaction) SH3C and Atx7 bind by nuclear magnetic resonance (View interaction) (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics; Cell Biology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics ; Cell Biology |
| 关键词[WOS] | HISTONE ACETYLTRANSFERASE ACTIVITY ; CBL-ASSOCIATED PROTEIN ; SIGNAL-TRANSDUCTION ; BINDING-PROTEIN ; GENE ; CAP ; CAP/PONSIN ; PEPTIDES ; AFFINITY ; INTERACT |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000324033700002 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/457]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Jiang, YJ,Zhou, CJ,Zhou, ZR,et al. Structural basis for recognition of the third SH3 domain of full-length R85 (R85FL)/ponsin by ataxin-7[J]. FEBS LETTERS,2013,587(18):2905-2911. |
| APA | Jiang, YJ,Zhou, CJ,Zhou, ZR,Wu, M,&Hu, HY.(2013).Structural basis for recognition of the third SH3 domain of full-length R85 (R85FL)/ponsin by ataxin-7.FEBS LETTERS,587(18),2905-2911. |
| MLA | Jiang, YJ,et al."Structural basis for recognition of the third SH3 domain of full-length R85 (R85FL)/ponsin by ataxin-7".FEBS LETTERS 587.18(2013):2905-2911. |
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