Disruption of disulfide restriction at integrin knees induces activation and ligand-independent signaling of alpha(4)beta(7)
文献类型：期刊论文
| 作者 | Zhang, K; Pan, YD; Qi, JP; Yue, J; Zhang, MB; Xu, CQ; Li, GH; Chen, JF |
| 刊名 | JOURNAL OF CELL SCIENCE
 |
| 出版日期 | 2013 |
| 卷号 | 126期号:21页码:5030-5041 |
| 关键词 | Disulfide-restriction Integrin clustering Ligand-independent signaling |
| 通讯作者 | Li, GH (reprint author), Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Mol React Dynam, Lab Mol Modeling & Design, Dalian 116023, Peoples R China.,ghli@dicp.ac.cn ; jfchen@sibcb.ac.cn |
| 英文摘要 | Control of integrin activation and signaling plays crucial roles in cell adhesion, spreading and migration. Here, we report that selective breakage of two conserved disulfide bonds located at the knees of integrin alpha(4)C589-C594 and beta(7)C494-C526 activated alpha(4)beta(7). This activated integrin had a unique structure that was different from the typical extended conformation of active integrin. In addition, these activated alpha(4)beta(7) integrins spontaneously clustered on the cell membrane and triggered integrin downstream signaling independent of ligand binding. Although these disulfide bonds were not broken during alpha(4)beta(7) activation by inside-out signaling or Mn2+, they could be specifically reduced by 0.1 mM dithiothreitol, a reducing strength that could be produced in vivo under certain conditions. Our findings reveal a novel mechanism of integrin activation under specific reducing conditions by which integrin can signal and promote cell spreading in the absence of ligand. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | CRYSTAL-STRUCTURE ; STRUCTURAL BASIS ; OUTSIDE-IN ; ALPHA-V-BETA-3 ; ADHESION ; DOMAINS ; ECTODOMAIN ; AFFINITY ; RECEPTOR ; ANTIBODY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000326392500022 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/465]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhang, K,Pan, YD,Qi, JP,et al. Disruption of disulfide restriction at integrin knees induces activation and ligand-independent signaling of alpha(4)beta(7)[J]. JOURNAL OF CELL SCIENCE,2013,126(21):5030-5041. |
| APA | Zhang, K.,Pan, YD.,Qi, JP.,Yue, J.,Zhang, MB.,...&Chen, JF.(2013).Disruption of disulfide restriction at integrin knees induces activation and ligand-independent signaling of alpha(4)beta(7).JOURNAL OF CELL SCIENCE,126(21),5030-5041. |
| MLA | Zhang, K,et al."Disruption of disulfide restriction at integrin knees induces activation and ligand-independent signaling of alpha(4)beta(7)".JOURNAL OF CELL SCIENCE 126.21(2013):5030-5041. |
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