The effect of the number of transferred embryos, the interval between nuclear transfer and embryo transfer, and the transfer pattern on pig cloning efficiency
文献类型：期刊论文
| 作者 | Rim, CH; Fu, ZX; Bao, L; Chen, HD; Zhang, D; Luo, Q; Ri, HC; Huang, HF; Luan, ZD; Zhang, Y |
| 刊名 | ANIMAL REPRODUCTION SCIENCE
 |
| 出版日期 | 2013 |
| 卷号 | 143期号:1页码:91-96 |
| 关键词 | Nuclear transfer Embryo transfer Culture time Single oviduct Double oviduct |
| 通讯作者 | Jong, UM (reprint author), Zhejiang Univ, Sch Med, Coll Anim Sci, Hangzhou 310058, Zhejiang, Peoples R China.,jonguimyong@aliyun.com |
| 英文摘要 | To improve the efficiency of producing cloned pigs, we investigated the influence of the number of transferred embryos, the culturing interval between nuclear transfer (NT) and embryo transfer, and the transfer pattern (single oviduct or double oviduct) on cloning efficiency. The results demonstrated that transfer of either 150-200 or more than 200 NT embryos compared to transfer of 100-150 embryos resulted in a significantly higher pregnancy rate (48 +/- 16, 50 +/- 16 vs. 29 +/- 5%, p < 0.05) and average litter size (4.1 +/- 2.3, 7 +/- 3.6 vs. 2.5 +/- 0.5). In vitro culture of reconstructed embryos for a longer time (40 h vs. 20 h) resulted in higher (p < 0.05) pregnancy rate (44 9 vs. 31 +/- 3%) and delivery rate (44 9 vs. 25 +/- 9%). Furthermore, double oviductal transfer dramatically increased pregnancy rate (83 6 vs. 27 + 8%, p < 0.05), delivery rate (75 2 vs. 27 + 8%, p < 0.05) and average litter size (6.5 +/- 2.8 vs. 2.6 +/- 1.2) compared to single oviductal transfer. Our study demonstrated that an improvement in pig cloning efficiency is achieved by adjusting the number and in vitro culture time of reconstructed embryos as well as the embryo transfer pattern. (C) 2013 Elsevier B.V. All rights reserved. |
| 学科主题 | Agriculture; Reproductive Biology |
| 类目[WOS] | Agriculture, Dairy & Animal Science ; Reproductive Biology |
| 关键词[WOS] | ADULT SOMATIC-CELLS ; CLONED PIGS ; UTERINE CAPACITY ; PORCINE EMBRYOS ; PREGNANCY ; FETAL ; SWINE ; MAINTENANCE ; INTEGRATION ; ACTIVATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000329381600013 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/485]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Rim, CH,Fu, ZX,Bao, L,et al. The effect of the number of transferred embryos, the interval between nuclear transfer and embryo transfer, and the transfer pattern on pig cloning efficiency[J]. ANIMAL REPRODUCTION SCIENCE,2013,143(1):91-96. |
| APA | Rim, CH.,Fu, ZX.,Bao, L.,Chen, HD.,Zhang, D.,...&Jong, UM.(2013).The effect of the number of transferred embryos, the interval between nuclear transfer and embryo transfer, and the transfer pattern on pig cloning efficiency.ANIMAL REPRODUCTION SCIENCE,143(1),91-96. |
| MLA | Rim, CH,et al."The effect of the number of transferred embryos, the interval between nuclear transfer and embryo transfer, and the transfer pattern on pig cloning efficiency".ANIMAL REPRODUCTION SCIENCE 143.1(2013):91-96. |
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