Immune adaptor ADAP in T cells regulates HIV-1 transcription and cell-cell viral spread via different co-receptors
文献类型：期刊论文
| 作者 | Wei, B; Han, L; Abbink, TEM; Groppelli, E; Lim, D; Thaker, YR; Gao, W; Zhai, RR; Wang, JH; Lever, A |
| 刊名 | RETROVIROLOGY
 |
| 出版日期 | 2013 |
| 卷号 | 10期号:1页码:101-101 |
| 关键词 | ADAP HIV-1 transcription HIV-1 transmission Integrin Virological synapse |
| 通讯作者 | Wang, HY (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Cell Biol, Shanghai, Peoples R China.,hongyanwang@sibcb.ac.cn ; cer51@cam.ac.uk |
| 英文摘要 | Background: Immune cell adaptor protein ADAP (adhesion and degranulation-promoting adaptor protein) mediates aspects of T-cell adhesion and proliferation. Despite this, a connection between ADAP and infection by the HIV-1 (human immunodeficiency virus-1) has not been explored. Results: In this paper, we show for the first time that ADAP and its binding to SLP-76 (SH2 domain-containing leukocyte protein of 76 kDa) regulate HIV-1 infection via two distinct mechanisms and co-receptors. siRNA down-regulation of ADAP, or expression of a mutant that is defective in associating to its binding partner SLP-76 (termed M12), inhibited the propagation of HIV-1 in T-cell lines and primary human T-cells. In one step, ADAP and its binding to SLP-76 were needed for the activation of NF-kappa B and its transcription of the HIV-1 long terminal repeat (LTR) in cooperation with ligation of co-receptor CD28, but not LFA-1. In a second step, the ADAP-SLP-76 module cooperated with LFA-1 to regulate conjugate formation between T-cells and dendritic cells or other T-cells as well as the development of the virological synapse (VS) and viral spread between immune cells. Conclusions: These findings indicate that ADAP regulates two steps of HIV-1 infection cooperatively with two distinct receptors, and as such, serves as a new potential target in the blockade of HIV-1 infection. |
| 学科主题 | Virology |
| 类目[WOS] | Virology |
| 关键词[WOS] | HUMAN-IMMUNODEFICIENCY-VIRUS ; NF-KAPPA-B ; VIROLOGICAL SYNAPSES ; SIGNALING PATHWAYS ; ANTIGEN RECEPTOR ; ACTIVATION ; PROTEIN ; TYPE-1 ; KINASE ; TRANSMISSION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000329041100001 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/487]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wei, B,Han, L,Abbink, TEM,et al. Immune adaptor ADAP in T cells regulates HIV-1 transcription and cell-cell viral spread via different co-receptors[J]. RETROVIROLOGY,2013,10(1):101-101. |
| APA | Wei, B.,Han, L.,Abbink, TEM.,Groppelli, E.,Lim, D.,...&Rudd, CE.(2013).Immune adaptor ADAP in T cells regulates HIV-1 transcription and cell-cell viral spread via different co-receptors.RETROVIROLOGY,10(1),101-101. |
| MLA | Wei, B,et al."Immune adaptor ADAP in T cells regulates HIV-1 transcription and cell-cell viral spread via different co-receptors".RETROVIROLOGY 10.1(2013):101-101. |
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