DDA3 associates with microtubule plus ends and orchestrates microtubule dynamics and directional cell migration
文献类型：期刊论文
| 作者 | Zhang, LY; Shao, HY; Zhu, TG; Xia, P; Wang, ZK; Liu, LF; Yan, MM; Hill, DL; Fang, GW; Chen, ZJ |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2013 |
| 卷号 | 3期号:1页码:1681-1681 |
| 通讯作者 | Yao, XB (reprint author), Anhui Key Lab Cellular Dynam, Hefei 230026, Peoples R China.,xyao@msm.edu |
| 英文摘要 | Cell motility and adhesion involve orchestrated interaction of microtubules (MTs) with their plus-end tracking proteins (+TIPs). However, the mechanisms underlying regulations of MT dynamics and directional cell migration are still elusive. Here, we show that DDA3-EB1 interaction orchestrates MT plus-end dynamics and facilitates directional cell migration. Biochemical characterizations reveal that DDA3 interacts with EB1 via its SxIP motif within the C-terminal Pro/Ser-rich region. Time-lapse and total internal reflection fluorescence (TIRF) microscopic assays demonstrate that DDA3 exhibits EB1-dependent, MT plus-end loading and tracking. The EB1-based loading of DDA3 is responsible for MT plus-ends stabilization at the cell cortex, which in turn orchestrates directional cell migration. Interestingly, the DDA3-EB1 interaction is potentially regulated by EB1 acetylation, which may account for physiological regulation underlying EGF-elicited cell migration. Thus, the EB1-based function of DDA3 links MT dynamics to directional cell migration. |
| 学科主题 | Science & Technology - Other Topics |
| 类目[WOS] | Multidisciplinary Sciences |
| 关键词[WOS] | ADENOMATOUS POLYPOSIS-COLI ; TIP LOCALIZATION SIGNAL ; TRACKING PROTEINS ; ACTIN-FILAMENTS ; EB1 ; IQGAP1 ; GROWTH ; CLASP2 ; CDC42 ; PHOSPHORYLATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000318537400001 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/489]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhang, LY,Shao, HY,Zhu, TG,et al. DDA3 associates with microtubule plus ends and orchestrates microtubule dynamics and directional cell migration[J]. SCIENTIFIC REPORTS,2013,3(1):1681-1681. |
| APA | Zhang, LY.,Shao, HY.,Zhu, TG.,Xia, P.,Wang, ZK.,...&Yao, XB.(2013).DDA3 associates with microtubule plus ends and orchestrates microtubule dynamics and directional cell migration.SCIENTIFIC REPORTS,3(1),1681-1681. |
| MLA | Zhang, LY,et al."DDA3 associates with microtubule plus ends and orchestrates microtubule dynamics and directional cell migration".SCIENTIFIC REPORTS 3.1(2013):1681-1681. |
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