The Application of SILAC Mouse in Human Body Fluid Proteomics Analysis Reveals Protein Patterns Associated with IgA Nephropathy
文献类型：期刊论文
| 作者 | Zhao, SL; Li, RX; Cai, XF; Chen, WJ; Li, QR; Xing, T; Zhu, WJ; Chen, YE; Zeng, R; Deng, YY |
| 刊名 | EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
 |
| 出版日期 | 2013 |
| 期号 | 1页码:275390-275390 |
| 通讯作者 | Zeng, R (reprint author), Chinese Acad Sci, Inst Biochem & Cell Biol, Key Lab Syst Biol, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China.,zr@sibs.ac.cn ; dengyueyi@medmail.com.cn |
| 英文摘要 | Body fluid proteome is the most informative proteome from a medical viewpoint. But the lack of accurate quantitation method for complicated body fluid limited its application in disease research and biomarker discovery. To address this problem, we introduced a novel strategy, in which SILAC-labeled mouse serum was used as internal standard for human serum and urine proteome analysis. The SILAC-labeled mouse serum was mixed with human serum and urine, and multidimensional separation coupled with tandem mass spectrometry (IEF-LC-MS/MS) analysis was performed. The shared peptides between two species were quantified by their SILAC pairs, and the human-only peptides were quantified by mouse peptides with coelution. The comparison for the results from two replicate experiments indicated the high repeatability of our strategy. Then the urine from Immunoglobulin A nephropathy patients treated and untreated was compared by this quantitation strategy. Fifty-three peptides were found to be significantly changed between two groups, including both known diagnostic markers for IgAN and novel candidates, such as Complement C3, Albumin, VDBP, ApoA, 1 and IGFBP7. In conclusion, we have developed a practical and accurate quantitation strategy for comparison of complicated human body fluid proteome. The results from such strategy could provide potential disease-related biomarkers for evaluation of treatment. |
| 学科主题 | Integrative & Complementary Medicine |
| 类目[WOS] | Integrative & Complementary Medicine |
| 关键词[WOS] | DIABETIC-NEPHROPATHY ; VITAMIN-D ; URINARY ALBUMIN ; COMPLEMENT C3 ; EXCRETION ; PROGRESSION ; PREDICTOR |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000319571500001 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/493]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhao, SL,Li, RX,Cai, XF,et al. The Application of SILAC Mouse in Human Body Fluid Proteomics Analysis Reveals Protein Patterns Associated with IgA Nephropathy[J]. EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE,2013(1):275390-275390. |
| APA | Zhao, SL.,Li, RX.,Cai, XF.,Chen, WJ.,Li, QR.,...&Deng, YY.(2013).The Application of SILAC Mouse in Human Body Fluid Proteomics Analysis Reveals Protein Patterns Associated with IgA Nephropathy.EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE(1),275390-275390. |
| MLA | Zhao, SL,et al."The Application of SILAC Mouse in Human Body Fluid Proteomics Analysis Reveals Protein Patterns Associated with IgA Nephropathy".EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE .1(2013):275390-275390. |
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