Serum Proteome Changes in Healthy Subjects with Different Genotypes of NOS1AP in the Chinese Population
文献类型：期刊论文
| 作者 | Jiang, F; Wang, CR; Li, RX; Sheng, QH; Hu, C; Zhang, R; Fang, QC; Bao, YQ; Xiang, KS; Zeng, R |
| 刊名 | JOURNAL OF DIABETES RESEARCH
 |
| 出版日期 | 2013 |
| 期号 | 1页码:357630-357630 |
| 通讯作者 | Jia, WP (reprint author), Shanghai Jiao Tong Univ, Affiliated Peoples Hosp 6, Shanghai Key Lab Diabet Mellitus,Shanghai Clin Ct, Dept Endocrinol & Metab,Shanghai Diabet Inst, 600 Yishan Rd, Shanghai 200233, Peoples R China.,wpjia@sjtu.edu.cn |
| 英文摘要 | Type 2 diabetes and its chronic complications have become a worldwide epidemic nowadays. However, its molecular mechanism is still unknown. We have previously identified a novel variant rs12742393 of NOS1AP for type 2 diabetes susceptibility in the Chinese population. In this study, we analyzed the total serum profiling among three genotypes of rs12742393 to discover potential crosstalk under the variant and the disease through proteomic analyses for the first time. We used OFFGEL peptide fractionation, LC-MS/MS analysis, and label-free quantification to profile the fasting human serum samples of the genotypes in rs12742393 (n = 4, for CC, AC, and AA, resp.). Four proteins were identified, including apoA4, alpha1-ACT, HABP2, and keratin 10, with blood levels changed significantly between CC and AA homozygotes of rs12742393. Compared with AA group, the levels of apoA4 increased (P = 0.000265), whereas the concentration of alpha1-ACT, HABP2, and keratin 10 decreased in CC group (P = 0.011116, 0.021175, and 0.015661, resp.). Then we selected additional fasting serum samples for ELISA and western blot validation. However, no significant differences were identified by neither ELISA nor western blot (P > 0.05). The protein profiling changes between the genotypes of rs12742393 indicated that this SNP might play a role in the development of type 2 diabetes. |
| 学科主题 | Endocrinology & Metabolism; Research & Experimental Medicine |
| 类目[WOS] | Endocrinology & Metabolism ; Medicine, Research & Experimental |
| 关键词[WOS] | NITRIC-OXIDE SYNTHASE ; INSULIN-SECRETION ; A-IV ; ACTIVATING PROTEASE ; BETA-CELLS ; FACTOR-VII ; GLUCOSE ; PLASMA ; GENE ; PKC |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000318672100001 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/495]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Jiang, F,Wang, CR,Li, RX,et al. Serum Proteome Changes in Healthy Subjects with Different Genotypes of NOS1AP in the Chinese Population[J]. JOURNAL OF DIABETES RESEARCH,2013(1):357630-357630. |
| APA | Jiang, F.,Wang, CR.,Li, RX.,Sheng, QH.,Hu, C.,...&Jia, WP.(2013).Serum Proteome Changes in Healthy Subjects with Different Genotypes of NOS1AP in the Chinese Population.JOURNAL OF DIABETES RESEARCH(1),357630-357630. |
| MLA | Jiang, F,et al."Serum Proteome Changes in Healthy Subjects with Different Genotypes of NOS1AP in the Chinese Population".JOURNAL OF DIABETES RESEARCH .1(2013):357630-357630. |
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