Interdomain communication modulates the tRNA-dependent pre-transfer editing of leucyl-tRNA synthetase
文献类型：期刊论文
| 作者 | Tan, M; Zhu, B; Liu, RJ; Chen, X; Zhou, XL; Wang, ED |
| 刊名 | BIOCHEMICAL JOURNAL
 |
| 出版日期 | 2013 |
| 卷号 | 449期号:1页码:123-131 |
| 关键词 | domain communication leucyl-tRNA synthetase (LeuRS) pre-transfer editing quality control tRNA |
| 通讯作者 | Wang, ED (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol,Ctr RNA Res, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,edwang@sibs.ac.cn |
| 英文摘要 | EcLeuRS [Escherichia coli LeuRS (leucyl-tRNA synthetase)] has evolved both tRNA-dependent pre- and post-transfer editing capabilities to ensure catalytic specificity. Both editing functions rely on the entry of the tRNA CCA tail into the editing domain of the LeuRS enzyme, which, according to X-ray crystal structural studies, leads to a dynamic disordered orientation of the interface between the synthetic and editing domains. The results of the present study show that this tRNA-triggered conformational rearrangement leads to interdomain communication between the editing and synthetic domains through their interface, and this communication mechanism modulates the activity of tRNA-dependent pre-transfer editing. Furthermore, tRNA-dependent editing reaction inhibits misactivating non-cognate amino acids from the synthetic active site. These results also suggested a novel quality control mechanism of EcLeuRS which is achieved through the co-ordination between the synthetic and editing domains. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | DOMAIN-DOMAIN COMMUNICATION ; GENETIC-CODE ; PROTEIN-SYNTHESIS ; CRYSTAL-STRUCTURE ; QUALITY-CONTROL ; CP1 DOMAIN ; COMPLEX ; AMINOACYLATION ; DISCRIMINATION ; MISTRANSLATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000312470800012 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/526]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Tan, M,Zhu, B,Liu, RJ,et al. Interdomain communication modulates the tRNA-dependent pre-transfer editing of leucyl-tRNA synthetase[J]. BIOCHEMICAL JOURNAL,2013,449(1):123-131. |
| APA | Tan, M,Zhu, B,Liu, RJ,Chen, X,Zhou, XL,&Wang, ED.(2013).Interdomain communication modulates the tRNA-dependent pre-transfer editing of leucyl-tRNA synthetase.BIOCHEMICAL JOURNAL,449(1),123-131. |
| MLA | Tan, M,et al."Interdomain communication modulates the tRNA-dependent pre-transfer editing of leucyl-tRNA synthetase".BIOCHEMICAL JOURNAL 449.1(2013):123-131. |
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