Detecting miRNAs by liquid hybridization and color development
文献类型：期刊论文
| 作者 | Li, XQ; Ni, MJ; Zhang, YL |
| 刊名 | METHODS
 |
| 出版日期 | 2012 |
| 卷号 | 58期号:2页码:151-155 |
| 关键词 | Method MicroRNA Liquid hybridization Avidin-biotin detection system Color development |
| 通讯作者 | Li, XQ (reprint author), Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai Key Lab Mol Androl, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,lixq@sibs.ac.cn ; ylzhang@sibs.ac.cn |
| 英文摘要 | Currently, two methods, PCR and Northern blot, are widely used to detect individual microRNAs (miRNA). Although PCR is highly sensitive, false positives and difficulties of primer design discourage its use. While a Northern blot is an effective tool, traditional Northern blot protocols are complicated, time-consuming, and usually inconvenient for users. Liquid Northern blot methods are rapid but require instruments for detection of fluorescent signals. Here, we describe an alternative protocol, liquid hybridization and color development (LHCD), based on the rapidity of liquid hybridization and the signal amplification of avidin-biotin complex (ABC) for detection. LHCD can distinguish a one-nucleotide difference within a miRNA family and allow for the sensitive detection of 2.5 f mol of miRNAs. Furthermore, LHCD is not only simple and rapid, but detection is visual and so it does not require expensive equipment. LHCD is easy to learn and convenient for miRNA analyses. (C) 2012 Elsevier Inc. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemical Research Methods ; Biochemistry & Molecular Biology |
| 关键词[WOS] | MICRORNA DETECTION ; C-ELEGANS ; BIOGENESIS ; LIN-14 ; PROBES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000316235300011 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/540]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Li, XQ,Ni, MJ,Zhang, YL. Detecting miRNAs by liquid hybridization and color development[J]. METHODS,2012,58(2):151-155. |
| APA | Li, XQ,Ni, MJ,&Zhang, YL.(2012).Detecting miRNAs by liquid hybridization and color development.METHODS,58(2),151-155. |
| MLA | Li, XQ,et al."Detecting miRNAs by liquid hybridization and color development".METHODS 58.2(2012):151-155. |
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