USE OF HUMAN AMNIOTIC EPITHELIAL CELLS AS A FEEDER LAYER TO SUPPORT UNDIFFERENTIATED GROWTH OF MOUSE SPERMATOGONIAL STEM CELLS VIA EPIGENETIC REGULATION OF THE Nanog AND Oct-4 PROMOTERS
文献类型：期刊论文
| 作者 | Liu, T; Huang, YY; Huang, Q; Jiang, LZ; Guo, LH; Liu, ZX |
| 刊名 | ACTA BIOLOGICA HUNGARICA
 |
| 出版日期 | 2012 |
| 卷号 | 63期号:2页码:167-179 |
| 关键词 | Human amnion epithelial cells mouse spermatogonial stem cells undifferentiated DNA methylation histone H3K4 trimethylation |
| 通讯作者 | Guo, LH (reprint author), Chinese Acad Sci, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China.,liute79@yahoo.com |
| 英文摘要 | Spermatogonial stem cells (SSCs) are defined by unique properties like other stem cells. However, there are two major challenges: long-term cultivation of normal SSCs into stable cell lines and maintaining the SSCs as undifferentiated and capable of self-renewal. Here, we compared different culture methods for mouse SSCs isolated and cultured from testicular tissue. We found that human amniotic epithelial cells (hAECs) can behave as feeder cells, allowing mouse SSCs to maintain a high level of alkaline phosphatase (AP) activity when cultured long-term. Also, we observed that expression of Nanog, Oct-4 and other important stem cells markers were higher in mouse SSCs cultured on hAECs compared to those cultured on MEF or without any feeder cells. Furthermore, we demonstrated that the CpG islands of the Nanog and Oct-4 promoters were hypomethylated in cells cultured on hAECs. In addition, mouse SSCs cultured on hAECs exhibited higher levels of H3AC and H3K4Me3 in the Nanog and Oct-4 promoters than those cultured on MEF or without feeder cells. Taken together, these results suggest that the hAEC-induced epigenetic modifications at the Nanog and Oct-4 locus could be a key mechanism for maintaining mouse SSCs in an undifferentiated state capable of self-renewal. |
| 学科主题 | Life Sciences & Biomedicine - Other Topics |
| 类目[WOS] | Biology |
| 关键词[WOS] | EMBRYONIC STEM ; DEVELOPMENTAL REGULATORS ; SOMATIC-CELLS ; ES CELLS ; PLURIPOTENCY ; GENERATION ; EXPRESSION ; TESTIS ; DIFFERENTIATION ; TRANSPLANTATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000306504800001 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/542]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Liu, T,Huang, YY,Huang, Q,et al. USE OF HUMAN AMNIOTIC EPITHELIAL CELLS AS A FEEDER LAYER TO SUPPORT UNDIFFERENTIATED GROWTH OF MOUSE SPERMATOGONIAL STEM CELLS VIA EPIGENETIC REGULATION OF THE Nanog AND Oct-4 PROMOTERS[J]. ACTA BIOLOGICA HUNGARICA,2012,63(2):167-179. |
| APA | Liu, T,Huang, YY,Huang, Q,Jiang, LZ,Guo, LH,&Liu, ZX.(2012).USE OF HUMAN AMNIOTIC EPITHELIAL CELLS AS A FEEDER LAYER TO SUPPORT UNDIFFERENTIATED GROWTH OF MOUSE SPERMATOGONIAL STEM CELLS VIA EPIGENETIC REGULATION OF THE Nanog AND Oct-4 PROMOTERS.ACTA BIOLOGICA HUNGARICA,63(2),167-179. |
| MLA | Liu, T,et al."USE OF HUMAN AMNIOTIC EPITHELIAL CELLS AS A FEEDER LAYER TO SUPPORT UNDIFFERENTIATED GROWTH OF MOUSE SPERMATOGONIAL STEM CELLS VIA EPIGENETIC REGULATION OF THE Nanog AND Oct-4 PROMOTERS".ACTA BIOLOGICA HUNGARICA 63.2(2012):167-179. |
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