中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Efficient Generation of Lens Progenitor Cells from Cataract Patient-Specific Induced Pluripotent Stem Cells

文献类型:期刊论文

作者Qiu, XD; Yang, J; Liu, TJ; Jiang, YX; Le, QH; Lu, Y
刊名PLOS ONE
出版日期2012
卷号7期号:3页码:e32612-e32612
通讯作者Qiu, XD (reprint author), Fudan Univ, Eye & ENT Hosp, Dept Ophthalmol, Shanghai 200433, Peoples R China.,luyi0705@gmail.com
英文摘要The development of a technique to induce the transformation of somatic cells to a pluripotent state via the ectopic expression of defined transcription factors was a transformational event in the field of regenerative medicine. The development of this technique also impacted ophthalmology, as patient-specific induced pluripotent stemcells (iPSCs) may be useful resources for some ophthalmological diseases. The lens is a key refractive element in the eye that focuses images of the visual world onto the retina. To establish a new model for drug screening to treat lens diseases and investigating lens aging and development, we examined whether human lens epithelial cells (HLECs) could be induced into iPSCs and if lens-specific differentiation of these cells could be achieved under defined chemical conditions. We first efficiently reprogrammed HLECs from age-related cataract patients to iPSCs with OCT-4, SOX-2, and KLF-4. The resulting HLEC-derived iPS (HLE-iPS) colonies were indistinguishable from human ES cells with respect to morphology, gene expression, pluripotent marker expression and their ability to generate all embryonic germ-cell layers. Next, we performed a 3-step induction procedure: HLE-iPS cells were differentiated into large numbers of lens progenitor-like cells with defined factors (Noggin, BMP and FGF2), and we determined that these cells expressed lens-specific markers (PAX6, SOX2, SIX3, CRYAB, CRYAA, BFSP1, and MIP). In addition, HLE-iPS-derived lens cells exhibited reduced expression of epithelial mesenchymal transition (EMT) markers compared with human embryonic stem cells (hESCs) and fibroblast-derived iPSCs. Our study describes a highly efficient procedure for generating lens progenitor cells from cataract patient HLEC-derived iPSCs. These patient-derived pluripotent cells provide a valuable model for studying the developmental and molecular biological mechanisms that underlie cell determination in lens development and cataract pathophysiology.
学科主题Science & Technology - Other Topics
类目[WOS]Multidisciplinary Sciences
关键词[WOS]POSTERIOR CAPSULE OPACIFICATION ; DEFINED CONDITIONS ; MOUSE ; DIFFERENTIATION ; ORGANIZATION ; FIBROBLASTS ; INDUCTION ; INSIGHTS ; SURGERY ; BIOLOGY
收录类别SCI
语种英语
WOS记录号WOS:000303017700077
版本出版稿
源URL[http://202.127.25.143/handle/331003/554]  
专题上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式
GB/T 7714
Qiu, XD,Yang, J,Liu, TJ,et al. Efficient Generation of Lens Progenitor Cells from Cataract Patient-Specific Induced Pluripotent Stem Cells[J]. PLOS ONE,2012,7(3):e32612-e32612.
APA Qiu, XD,Yang, J,Liu, TJ,Jiang, YX,Le, QH,&Lu, Y.(2012).Efficient Generation of Lens Progenitor Cells from Cataract Patient-Specific Induced Pluripotent Stem Cells.PLOS ONE,7(3),e32612-e32612.
MLA Qiu, XD,et al."Efficient Generation of Lens Progenitor Cells from Cataract Patient-Specific Induced Pluripotent Stem Cells".PLOS ONE 7.3(2012):e32612-e32612.

入库方式: OAI收割

来源:上海生物化学与细胞生物学研究所

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