Acetylcholinesterase is associated with apoptosis in cells and contributes to insulin-dependent diabetes mellitus pathogenesis
文献类型：期刊论文
| 作者 | Zhang, B; Yang, L; Yu, LY; Lin, B; Hou, YN; Wu, J; Huang, Q; Han, YF; Guo, LH; Ouyang, Q |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2012 |
| 卷号 | 44期号:3页码:207-216 |
| 关键词 | diabetes acetylcholinesterase apoptosis cell streptozotocin |
| 通讯作者 | Zhang, XJ (reprint author), Chinese Acad Sci, Grad Student Sch, Shanghai Inst Biol Sci, State Key Lab Cell Biol,Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.,xjzhang@sibs.ac.cn |
| 英文摘要 | Acetylcholinesterase (AChE) expression is pivotal during apoptosis. Indeed, AChE inhibitors partially protect cells from apoptosis. Insulin-dependent diabetes mellitus (IDDM) is characterized in part by pancreatic -cell apoptosis. Here, we investigated the role of AChE in the development of IDDM and analyzed protective effects of AChE inhibitors. Multiple low-dose streptozotocin (MLD-STZ) administration resulted in IDDM in a mouse model. Western blot analysis, cytochemical staining, and immunofluorescence staining were used to detect AChE expression in MIN6 cells, primary cells, and apoptotic pancreatic cells of MLD-STZ-treated mice. AChE inhibitors were administered intraperitoneally to the MLD-STZ mice for 30 days. Blood glucose, plasma insulin, and creatine levels were measured, and glucose tolerance tests were performed. The effects of AChE inhibitors on MIN6 cells were also evaluated. AChE expression was induced in the apoptotic MIN6 cells and primary cells in vitro and pancreatic islets in vivo when treated with STZ. Induction and progressive accumulation of AChE in the pancreatic islets were associated with apoptotic cells during IDDM development. The administration of AChE inhibitors effectively decreased hyperglycemia and incidence of diabetes, and restored plasma insulin levels and plasma creatine clearance in the MLD-STZ mice. AChE inhibitors partially protected MIN6 cells from the damage caused by STZ treatment. Induction and accumulation of AChE in pancreatic islets and the protective effects of AChE inhibitors on the onset and development of IDDM indicate a close relationship between AChE and IDDM. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | ALZHEIMERS-DISEASE ; CHOLINERGIC SYSTEM ; EXPRESSION ; INHIBITORS ; STREPTOZOTOCIN ; ACHE ; ENZYMES ; BRAIN ; MODEL |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000300727400003 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/563]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhang, B,Yang, L,Yu, LY,et al. Acetylcholinesterase is associated with apoptosis in cells and contributes to insulin-dependent diabetes mellitus pathogenesis[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2012,44(3):207-216. |
| APA | Zhang, B.,Yang, L.,Yu, LY.,Lin, B.,Hou, YN.,...&Zhang, XJ.(2012).Acetylcholinesterase is associated with apoptosis in cells and contributes to insulin-dependent diabetes mellitus pathogenesis.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,44(3),207-216. |
| MLA | Zhang, B,et al."Acetylcholinesterase is associated with apoptosis in cells and contributes to insulin-dependent diabetes mellitus pathogenesis".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 44.3(2012):207-216. |
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