Human tetraspanin transmembrane 4 superfamily member 4 or intestinal and liver tetraspan membrane protein is overexpressed in hepatocellular carcinoma and accelerates tumor cell growth
文献类型：期刊论文
| 作者 | Li, Y; Wang, LM; Qiu, J; Da, L; Tiollais, P; Li, ZP; Zhao, MJ |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2012 |
| 卷号 | 44期号:3页码:224-232 |
| 关键词 | TM4SF4 il-TMP overexpression hepatocellular carcinima proliferation colony formation |
| 通讯作者 | Zhao, MJ (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,mjzhao@sibs.ac.cn |
| 英文摘要 | The human transmembrane 4 superfamily member 4 or intestinal and liver tetraspan membrane protein (TM4SF4/il-TMP) was originally cloned as an intestinal and liver tetraspan membrane protein and mediates density-dependent cell proliferation. The rat homolog of TM4SF4 was found to be up-regulated in regenerating liver after two-thirds hepatectomy and overexpression of TM4SF4 could enhance liver injury induced by CCl4. However, the expression and significance of TM4SF4/il-TMP in liver cancer remain unknown. Here, we report that TM4SF4/il-TMP is frequently and significantly overexpressed in hepatocellular carcinoma (HCC). Real-time quantitative reverse transcription polymerase chain reaction (RT-PCR) and western blot analysis showed that TM4SF4/il-TMP mRNA and protein levels were up-regulated in approximate to 80 of HCC tissues. Immunohistochemical analysis of a 75 paired HCC tissue microarray revealed that TM4SF4/il-TMP was significantly overexpressed in HCC tissues (P 0.001), and high immunointensity of TM4SF4/il-TMP tended to be in well-to-moderately differentiated HCC compared with poorly differentiated tumors. Functional studies showed that overexpression of TM4SF4/il-TMP in QGY-7701 and BEL-7404 HCC cell lines through stable transfection of TM4SF4 expression plasmid significantly promoted both cell growth and colony formation of HCC cells. Reduction of TM4SF4/il-TMP expression in QGY-7701 and BEL-7404 cells by stably transfecting TM4SF4 antisense plasmid caused great inhibition of cell proliferation. Our findings suggest that TM4SF4/il-TMP has the potential to be biomarker in HCC and plays a crucial role in promotion of cancer cell proliferation. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | LUNG-CANCER ; MOLECULAR-CLONING ; EXPRESSION ; IDENTIFICATION ; METASTASIS ; CDNA ; TM4SF5 ; CD151 ; GENE ; L6 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000300727400005 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/564]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Li, Y,Wang, LM,Qiu, J,et al. Human tetraspanin transmembrane 4 superfamily member 4 or intestinal and liver tetraspan membrane protein is overexpressed in hepatocellular carcinoma and accelerates tumor cell growth[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2012,44(3):224-232. |
| APA | Li, Y.,Wang, LM.,Qiu, J.,Da, L.,Tiollais, P.,...&Zhao, MJ.(2012).Human tetraspanin transmembrane 4 superfamily member 4 or intestinal and liver tetraspan membrane protein is overexpressed in hepatocellular carcinoma and accelerates tumor cell growth.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,44(3),224-232. |
| MLA | Li, Y,et al."Human tetraspanin transmembrane 4 superfamily member 4 or intestinal and liver tetraspan membrane protein is overexpressed in hepatocellular carcinoma and accelerates tumor cell growth".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 44.3(2012):224-232. |
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