MiR-200a is involved in rat epididymal development by targeting -catenin mRNA
文献类型：期刊论文
| 作者 | Wu, XJ; Zhao, BT; Li, W; Chen, Y; Liang, RQ; Li, L; Jin, YX; Ruan, KC |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2012 |
| 卷号 | 44期号:3页码:233-240 |
| 关键词 | miRNA microarray template switching miR-200a -catenin rat epididymis |
| 通讯作者 | Jin, YX (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,yxjin@sunm.shcnc.ac.cn ; kcruan@sibs.ac.cn |
| 英文摘要 | The expression of 350 microRNAs (miRNAs) in epididymis of rat from postnatal development to adult (from postnatal days 770) was profiled with home-made miRNA microarray. Among them, 48 miRNAs changed significantly, in which the expression of miR-200a increased obviously with time, in a good agreement with that obtained from northern blot analysis. The real-time quantitative-polymerase chain reaction result indicated that temporal expression of rat -catenin was exactly inversed to that of miR-200a during rat epididymal development, implying that miR-200a might also target -catenin mRNA in rat epididymis as reported by Saydam et al. in humans. The bioinformatic analysis indicated that 3 untranslated region of rat -catenin mRNA did contain a putative binding site for miR-200a. Meanwhile, it was found that the sequence of this binding site was different from that of human -catenin mRNA with a deletion of two adjacent nucleotides (U and C). But the results of luciferase targeting assay in HEK 293T cells and the overexpression of miR-200a in rat NRK cells demonstrated that miR-200a did target rat -catenin mRNA and cause the suppression of its expression. All these results show that miR-200a should be involved in rat epididymal development by targeting -catenin mRNA of rat and suppressing its expression. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | E-CADHERIN ; MESENCHYMAL TRANSITION ; REPRESSORS ZEB1 ; EXPRESSION ; FAMILY ; CANCER |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000300727400006 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/565]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wu, XJ,Zhao, BT,Li, W,et al. MiR-200a is involved in rat epididymal development by targeting -catenin mRNA[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2012,44(3):233-240. |
| APA | Wu, XJ.,Zhao, BT.,Li, W.,Chen, Y.,Liang, RQ.,...&Ruan, KC.(2012).MiR-200a is involved in rat epididymal development by targeting -catenin mRNA.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,44(3),233-240. |
| MLA | Wu, XJ,et al."MiR-200a is involved in rat epididymal development by targeting -catenin mRNA".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 44.3(2012):233-240. |
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