Splice blocking of zygotic sox31 leads to developmental arrest shortly after Mid-Blastula Transition and induces apoptosis in zebrafish
文献类型：期刊论文
| 作者 | Hu, SN; Yu, H; Zhang, YB; Wu, ZL; Yan, YC; Li, YX; Li, YY; Li, YP |
| 刊名 | FEBS LETTERS
 |
| 出版日期 | 2012 |
| 卷号 | 586期号:3页码:222-228 |
| 关键词 | sox31 MBT Apoptosis |
| 通讯作者 | Li, YP (reprint author), Chinese Acad Sci, State Key Lab Cell Biol, Shanghai Key Lab Mol Androl, Shanghai Inst Biol Sci,Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.,Yipingli@sibs.ac.cn |
| 英文摘要 | Here we report that splice blocking morpholinos (Sb MO) against zebrafish sox31 elicit developmental arrest, likely through creating a series of dominant negative splicing variants. Embryos injected with the Sb MO develop normally before the Mid-Blastula Transition (MBT); however, they do not initiate epiboly. Microarray analysis of mRNAs collected at the dome stage revealed that the Sb MO impairs activation of a large set of zygotic genes and reduces degradation of maternal mRNA during MBT. Furthermore, an apoptotic response occurs in Sb morphants at about 6 hpf. SoxB1 family genes including sox31 thus play an essential role for early embryos traversing the transitional stage. (C) 2011 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics; Cell Biology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics ; Cell Biology |
| 关键词[WOS] | MIDBLASTULA TRANSITION ; GENE ; EXPRESSION ; TRANSCRIPTION ; ACTIVATION ; EMBRYOS ; MOUSE ; STAGE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000300186900004 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/566]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Hu, SN,Yu, H,Zhang, YB,et al. Splice blocking of zygotic sox31 leads to developmental arrest shortly after Mid-Blastula Transition and induces apoptosis in zebrafish[J]. FEBS LETTERS,2012,586(3):222-228. |
| APA | Hu, SN.,Yu, H.,Zhang, YB.,Wu, ZL.,Yan, YC.,...&Li, YP.(2012).Splice blocking of zygotic sox31 leads to developmental arrest shortly after Mid-Blastula Transition and induces apoptosis in zebrafish.FEBS LETTERS,586(3),222-228. |
| MLA | Hu, SN,et al."Splice blocking of zygotic sox31 leads to developmental arrest shortly after Mid-Blastula Transition and induces apoptosis in zebrafish".FEBS LETTERS 586.3(2012):222-228. |
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