A Redox-Sensitive Luciferase Assay for Determining the Localization and Topology of Endoplasmic Reticulum Proteins
文献类型：期刊论文
| 作者 | Li, HY; Zheng, XM; Che, MX; Hu, HY |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2012 |
| 卷号 | 7期号:4页码:e35628-e35628 |
| 通讯作者 | Li, HY (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai, Peoples R China.,hyhu@sibs.ac.cn |
| 英文摘要 | Correct localization and transmembrane topology are crucial for the proteins residing and functioning in the endoplasmic reticulum (ER). We have developed a rapid and convenient assay, based on the redox-sensitive luciferase from Gaussia princeps (Gluc) and green fluorescence protein (GFP), to determine the localization or topology of ER proteins. Using the tandem Gluc-GFP reporter fused to different positions of a target protein, we successfully characterized the topologies of two ER transmembrane proteins Herp and HRD1 that are involved in the ER quality control system. This assay method may also be applicable to the proteins in secretory pathway, plasma membrane, and other compartments of cells. |
| 学科主题 | Science & Technology - Other Topics |
| 类目[WOS] | Multidisciplinary Sciences |
| 关键词[WOS] | GREEN FLUORESCENT PROTEIN ; GAUSSIA LUCIFERASE ; SECRETORY PATHWAY ; QUALITY-CONTROL ; DEGRADATION ; STRESS ; DOMAIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000305350600106 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/569]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Li, HY,Zheng, XM,Che, MX,et al. A Redox-Sensitive Luciferase Assay for Determining the Localization and Topology of Endoplasmic Reticulum Proteins[J]. PLOS ONE,2012,7(4):e35628-e35628. |
| APA | Li, HY,Zheng, XM,Che, MX,&Hu, HY.(2012).A Redox-Sensitive Luciferase Assay for Determining the Localization and Topology of Endoplasmic Reticulum Proteins.PLOS ONE,7(4),e35628-e35628. |
| MLA | Li, HY,et al."A Redox-Sensitive Luciferase Assay for Determining the Localization and Topology of Endoplasmic Reticulum Proteins".PLOS ONE 7.4(2012):e35628-e35628. |
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