Highly Efficient Derivation of Skeletal Myotubes from Human Embryonic Stem Cells
文献类型：期刊论文
| 作者 | Rao, LJ; Tang, WJ; Wei, YZ; Bao, L; Chen, JJ; Chen, HD; He, LXZ; Lu, PF; Ren, JT; Wu, L |
| 刊名 | STEM CELL REVIEWS AND REPORTS
 |
| 出版日期 | 2012 |
| 卷号 | 8期号:4页码:1109-1119 |
| 关键词 | Human embryonic stem cells Skeletal myotube Directed differentiation Muscle development Tet-on MyoD |
| 通讯作者 | Xiao, L (reprint author), Zhejiang Univ, Stem Cell & Dev Biol Res Ctr, Coll Anim Sci, 866 Yuhangtang Rd,Nongshenghuan Bldg E,Room 301, Hangzhou 310058, Zhejiang, Peoples R China.,leixiao@sibs.ac.cn |
| 英文摘要 | Human embryonic stem cells (hESCs) are a promising model for the research of embryonic development and regenerative medicine. Since the first hESC line was established, many researchers have shown that pluripotent hESCs can be directed into many types of functional adult cells in culture. However, most of the reported methods have induced differentiation through the alteration of growth factors in the culture medium. These methods are time consuming; moreover, it is difficult to obtain a pure population of the desired cells because of the low efficiency of induction. In this study, we used a lentiviral-based inducible gene-expression system in hESCs to control the ectopic expression of MyoD, which is an essential transcription factor in skeletal muscle development. The induction of MyoD can efficiently direct the pluripotent hESCs into mesoderm in 24 h. The cells then become proliferated myoblasts and finally form multinucleated myotubes in vitro. The whole procedure took about 10 days, with an induction efficiency of over 90 %. To our knowledge, this is the first time that hESCs have been induced into terminally differentiated cells with only one factor. In the future, these results could be a potential resource for cell therapy for diseases of muscle dysfunction. |
| 学科主题 | Cell Biology; Research & Experimental Medicine |
| 类目[WOS] | Cell & Tissue Engineering ; Cell Biology ; Medicine, Research & Experimental |
| 关键词[WOS] | SATELLITE CELLS ; IN-VITRO ; TERMINAL DIFFERENTIATION ; TRANSGENE EXPRESSION ; HUMAN ES ; MUSCLE ; GENE ; MYOD ; CULTURE ; MOUSE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000311510600009 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/571]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Rao, LJ,Tang, WJ,Wei, YZ,et al. Highly Efficient Derivation of Skeletal Myotubes from Human Embryonic Stem Cells[J]. STEM CELL REVIEWS AND REPORTS,2012,8(4):1109-1119. |
| APA | Rao, LJ.,Tang, WJ.,Wei, YZ.,Bao, L.,Chen, JJ.,...&Xiao, L.(2012).Highly Efficient Derivation of Skeletal Myotubes from Human Embryonic Stem Cells.STEM CELL REVIEWS AND REPORTS,8(4),1109-1119. |
| MLA | Rao, LJ,et al."Highly Efficient Derivation of Skeletal Myotubes from Human Embryonic Stem Cells".STEM CELL REVIEWS AND REPORTS 8.4(2012):1109-1119. |
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