Molecular cloning and identification of mouse epididymis-specific gene mHong1, the homologue of rat HongrES1
文献类型：期刊论文
| 作者 | Hu, SG; Du, H; Yao, GX; Zhang, YL |
| 刊名 | ASIAN JOURNAL OF ANDROLOGY
 |
| 出版日期 | 2012 |
| 卷号 | 14期号:4页码:626-634 |
| 关键词 | androgen epididymal secretory protein epididymis glycosylation sperm maturation |
| 通讯作者 | Zhang, YL (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai Key Lab Mol Androl,State Key Lab Mol Bio, Shanghai 200031, Peoples R China.,ylzhang@sibs.ac.cn |
| 英文摘要 | Previous studies have shown that rat epididymis-specific gene HongrES1 plays important roles in sperm capacitation and fertility. In this study, we cloned the mouse homologue gene by sequence alignment and RT-PCR methods and designated it as mHong1. The mHong1 gene is located on chromosome 12p14, spanning five exons. The cDNA sequence consists of 1257 nucleotides and encodes a 419 amino-acid protein with a predicted N-terminal signal peptide of 20 amino acids. The mHong1 mRNA shows similarity with HongrES1 in the expression patterns: (i) specific expression in epididymal tissue, especially in the cauda region; and (ii) androgen-dependence but testicular fluid factor independence. Its protein product shows 71% similarity with HongrES1 and contains a classical serpin domain as does HongrES1. A polyclonal antibody against mHong1 with high specificity and sensitivity was raised. Like HongrES1, the mHong1 protein shows a checker-board expression pattern in the epididymal epithelium and is secreted into the epididymal lumen. The mHong1 protein shows higher glycosylation than HongrES1. Although both of them are deposited onto the sperm head surface, mHong1 is localized to the equatorial segment, which is different from that of HongrES1. The mHong1 protein can be removed from the sperm membrane by high ionic strength and therefore can be classed as an extrinsic membrane protein. Collectively, we conclude that mHong1 is the homologue of HongrES1 and the present work paves the way for establishing animal models to elucidate the precise functions of HongrES1 and mHong1. Asian Journal of Andrology (2012) 14, 626-634; doi:10.1038/aja.2011.176; published online 19 March 2012 |
| 学科主题 | Endocrinology & Metabolism; Urology & Nephrology |
| 类目[WOS] | Andrology ; Urology & Nephrology |
| 关键词[WOS] | POLYCLONAL ANTISERA ; SPERM ; PROTEIN ; EXPRESSION ; CAPACITATION ; MEMBER ; SUPERFAMILY ; MATURATION ; MEMBRANE ; BINDING |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000305998300024 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/575]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Hu, SG,Du, H,Yao, GX,et al. Molecular cloning and identification of mouse epididymis-specific gene mHong1, the homologue of rat HongrES1[J]. ASIAN JOURNAL OF ANDROLOGY,2012,14(4):626-634. |
| APA | Hu, SG,Du, H,Yao, GX,&Zhang, YL.(2012).Molecular cloning and identification of mouse epididymis-specific gene mHong1, the homologue of rat HongrES1.ASIAN JOURNAL OF ANDROLOGY,14(4),626-634. |
| MLA | Hu, SG,et al."Molecular cloning and identification of mouse epididymis-specific gene mHong1, the homologue of rat HongrES1".ASIAN JOURNAL OF ANDROLOGY 14.4(2012):626-634. |
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