Murine embryonic stem cell-derived hepatocytes correct metabolic liver disease after serial liver repopulation
文献类型：期刊论文
| 作者 | He, ZY; Deng, L; Li, YF; Xiang, D; Hu, JK; Chen, YX; Wang, MJ; Chen, F; Liu, CC; Li, WL |
| 刊名 | INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
 |
| 出版日期 | 2012 |
| 卷号 | 44期号:4页码:648-658 |
| 关键词 | Embryonic stem cell Hepatocyte Metabolic function Liver repopulation Cell transplantation |
| 通讯作者 | Wang, X (reprint author), Second Mil Med Univ, Dept Cell Biol, 800 Xiangyin Rd, Shanghai 200433, Peoples R China.,yphu@smmu.edu.cn ; wangx@sibs.ac.cn |
| 英文摘要 | Although embryonic stem (ES) cell-derived hepatocytes have the capacity for liver engraftment and repopulation, their in vivo hepatic function has not been analyzed yet. We aimed to determine the metabolic function and therapeutic action of ES cell-derived hepatocytes after serial liver repopulations in fumaryl acetoacetate hydrolase knockout (Fah(-/-)) mice. Albumin expressing (Alb(+)) cells were obtained by hepatic differentiation of ES cells using two frequently reported methods. After transplantation, variable levels of liver repopulation were found in Fah(-/-) mice recipients. FAH expressing (FAH) hepatocytes were found either as single cells or as nodules with multiple hepatocytes. After serial transplantation, the proportion of the liver that was repopulated by the re-transplanted FAH(+) hepatocytes increased significantly. ES cell-derived FAH. hepatocytes were found in homogenous nodules and corrected the liver metabolic disorder of Fah(-/-) recipients and rescued them from death. ES cell-derived hepatocytes had normal karyotype, hepatocytic morphology and metabolic function both in vitro and in vivo. In conclusion. ES cell-derived hepatocytes were capable of liver repopulation and correction of metabolic defects after serial transplantation. Our results are an important piece of evidence to support future clinical applications of ES cell-derived hepatocytes in treating liver diseases. (C) 2012 Elsevier Ltd. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Cell Biology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Cell Biology |
| 关键词[WOS] | TYROSINEMIA TYPE-I ; MOUSE ES CELLS ; PROGENITOR CELLS ; FUNCTIONAL HEPATOCYTES ; DIFFERENTIATION ; ENDODERM ; TRANSPLANTATION ; MICE ; VIVO ; SPECIFICATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000302516300012 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/578]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | He, ZY,Deng, L,Li, YF,et al. Murine embryonic stem cell-derived hepatocytes correct metabolic liver disease after serial liver repopulation[J]. INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY,2012,44(4):648-658. |
| APA | He, ZY.,Deng, L.,Li, YF.,Xiang, D.,Hu, JK.,...&Wang, X.(2012).Murine embryonic stem cell-derived hepatocytes correct metabolic liver disease after serial liver repopulation.INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY,44(4),648-658. |
| MLA | He, ZY,et al."Murine embryonic stem cell-derived hepatocytes correct metabolic liver disease after serial liver repopulation".INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY 44.4(2012):648-658. |
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