Identification of a NodD repressible gene adjacent to nodM in Rhizobium leguminosarum biovar viciae
文献类型：期刊论文
| 作者 | Yang, XE; Hou, BH; Zong, CZ; Hong, GF |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2012 |
| 卷号 | 44期号:4页码:323-329 |
| 关键词 | nodM px3 2D gel electrophoresis protein difference |
| 通讯作者 | Hong, GF (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,gfhong@sibs.ac.cn |
| 英文摘要 | The nodFEL and nodMNT operons in Rhizobium leguminosarum biovar viciae are transcribed in the same orientation and induced by NodD in response to flavonoids secreted by legumes. In the narrow intergenic region between nodFEL and nodMNT, we identified a small gene divergently transcribed from nodM to the 3 end of nodL. Unlike the promoters upstream of nodF and nodM, the promoter of this gene is constitutively expressed. It appeared that its promoter might partially overlap with that of nodM and its expression was repressed by nodD. A deletion mutation was made and proteins produced by the mutant were compared with those by wild-type using 2D gel electrophoresis. Several protein differences were identified suggesting that this small gene influences the expression or stability of these proteins. However, the mutant nodulated its host plant (pea) normally. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | BROAD HOST RANGE ; NODULATION GENES ; PROTEIN ; REGION ; CONSERVATION ; EXUDATE ; ENCODES ; BINDING ; SYSTEM |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000302297500006 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/580]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Yang, XE,Hou, BH,Zong, CZ,et al. Identification of a NodD repressible gene adjacent to nodM in Rhizobium leguminosarum biovar viciae[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2012,44(4):323-329. |
| APA | Yang, XE,Hou, BH,Zong, CZ,&Hong, GF.(2012).Identification of a NodD repressible gene adjacent to nodM in Rhizobium leguminosarum biovar viciae.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,44(4),323-329. |
| MLA | Yang, XE,et al."Identification of a NodD repressible gene adjacent to nodM in Rhizobium leguminosarum biovar viciae".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 44.4(2012):323-329. |
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