PEDV ORF3 encodes an ion channel protein and regulates virus production
文献类型：期刊论文
| 作者 | Wang, K; Lu, W; Chen, JF; Xie, SQ; Shi, HY; Hsu, HJ; Yu, WJ; Xu, K; Bian, C; Fischer, WB |
| 刊名 | FEBS LETTERS
 |
| 出版日期 | 2012 |
| 卷号 | 586期号:4页码:384-391 |
| 关键词 | PEDV ORF3 Ion channel Molecular dynamics |
| 通讯作者 | Feng, L (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Pasteur Shanghai, Key Lab Mol Virol & Immunol, 225 S Chongqing Rd, Shanghai 200025, Peoples R China.,fl@hvri.ac.cn ; bsun@sibs.ac.cn |
| 英文摘要 | Several studies suggest that the open reading frame 3 (ORF3) gene of porcine epidemic diarrhea virus (PEDV) is related to viral infectivity and pathogenicity, but its function remains unknown. Here, we propose a structure model of the ORF3 protein consisting of four TM domains and forming a tetrameric assembly. ORF3 protein can be detected in PEDV-infected cells and it functions as an ion channel in both Xenopus laevis oocytes and yeast. Mutation analysis showed that Tyr170 in TM4 is important for potassium channel activity. Furthermore, viral production is reduced in infected Vero cells when ORF3 gene is silenced by siRNA. Interestingly, the ORF3 gene from an attenuated PEDV encodes a truncated protein with 49 nucleotide deletions, which lacks the ion channel activity. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics; Cell Biology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics ; Cell Biology |
| 关键词[WOS] | PORCINE EPIDEMIC DIARRHEA ; CORONAVIRUS ENVELOPE PROTEIN ; SEQUENCE-ANALYSIS ; 3A PROTEIN ; GENOME ; INTERFERENCE ; REPLICATION ; VIROPORINS ; EXPRESSION ; PREDICTION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000300588600017 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/586]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wang, K,Lu, W,Chen, JF,et al. PEDV ORF3 encodes an ion channel protein and regulates virus production[J]. FEBS LETTERS,2012,586(4):384-391. |
| APA | Wang, K.,Lu, W.,Chen, JF.,Xie, SQ.,Shi, HY.,...&Sun, B.(2012).PEDV ORF3 encodes an ion channel protein and regulates virus production.FEBS LETTERS,586(4),384-391. |
| MLA | Wang, K,et al."PEDV ORF3 encodes an ion channel protein and regulates virus production".FEBS LETTERS 586.4(2012):384-391. |
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