Secretome-Derived Isotope Tags (SDIT) Reveal Adipocyte-Derived Apolipoprotein C-I as a Predictive Marker for Cardiovascular Disease
文献类型：期刊论文
| 作者 | Li, RX; Ding, YB; Zhao, SL; Xiao, YY; Li, QR; Xia, FY; Sun, L; Lin, X; Wu, JR; Liao, K |
| 刊名 | JOURNAL OF PROTEOME RESEARCH
 |
| 出版日期 | 2012 |
| 卷号 | 11期号:5页码:2851-2862 |
| 关键词 | secretome-derived isotope tags adipocyte-secreted proteins obesity apolipoprotein C-I cardiovascular risk factors |
| 通讯作者 | Zeng, R (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Key Lab Syst Biol, Shanghai 200031, Peoples R China.,kliao@sibs.ac.cn ; zr@sibs.ac.cn |
| 英文摘要 | We developed a quantitative strategy, named secretome-derived isotopic tag (SDIT), to concurrently identify and quantify the adipocyte-secreted plasma proteins from normal and high-fat-diet (HFD) induced obese mice, based on the application of isotope-labeled secreted proteins from cultured mouse adipocytes as internal standards. We detected 197 proteins with significant changes between normal and obese mice plasma. Importantly, a novel adipocyte-secreted plasma protein, apolipoprotein C-I (apoC-I), significantly increased in the obese mice plasma. The expression and secretion of adipocyte apoC-I was detected in differentiated 3T3-L1 and primary rat adipocytes. Our in vitro experiments proved that functional Golgi apparatus was required for apoC-I secretion. Additionally, obese mice had increased apoC-I production in adipose tissue. Population survey of 367 participants showed that the plasma level of apoC-I was significantly increased in obese individuals compared with healthy individuals. After multiple adjustments for age and sex, the odds ratios for risk factors of cardiovascular disease including high LDL cholesterol, hypercholesterolemia, and hypertriglyceridemia, respectively, were used to compare the highest with the lowest apoC-I quartile. Taken together, our studies provide a novel strategy to concurrently identify and quantify tissue-specific secreted proteins. This strategy can be used to identify the largest global characterization of adipocyte-derived plasma proteome and provides a potential disease-related biomarker for clinical diagnoses. By selectively analyzing adipocyte-secreted proteins in plasma from obese vs lean murine and/or human subjects, we discovered that apoC-I is an adipocyte-secreted plasma protein and a predictive marker for cardiovascular disease. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemical Research Methods |
| 关键词[WOS] | ESTER TRANSFER PROTEIN ; ADIPOSE-TISSUE ; APOC-I ; METABOLIC SYNDROME ; MASS-SPECTROMETRY ; SEVERE HYPERTRIGLYCERIDEMIA ; LINKAGE DISEQUILIBRIUM ; TRANSGENIC MICE ; PROTEOMICS ; DIFFERENTIATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000303492100019 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/595]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Li, RX,Ding, YB,Zhao, SL,et al. Secretome-Derived Isotope Tags (SDIT) Reveal Adipocyte-Derived Apolipoprotein C-I as a Predictive Marker for Cardiovascular Disease[J]. JOURNAL OF PROTEOME RESEARCH,2012,11(5):2851-2862. |
| APA | Li, RX.,Ding, YB.,Zhao, SL.,Xiao, YY.,Li, QR.,...&Zeng, R.(2012).Secretome-Derived Isotope Tags (SDIT) Reveal Adipocyte-Derived Apolipoprotein C-I as a Predictive Marker for Cardiovascular Disease.JOURNAL OF PROTEOME RESEARCH,11(5),2851-2862. |
| MLA | Li, RX,et al."Secretome-Derived Isotope Tags (SDIT) Reveal Adipocyte-Derived Apolipoprotein C-I as a Predictive Marker for Cardiovascular Disease".JOURNAL OF PROTEOME RESEARCH 11.5(2012):2851-2862. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。