NRSF/REST is required for gastrulation and neurogenesis during zebrafish development
文献类型：期刊论文
| 作者 | Wang, XS; Ren, JK; Wang, ZG; Yao, JH; Fei, J |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2012 |
| 卷号 | 44期号:5页码:385-393 |
| 关键词 | NRSF REST in situ hybridization morpholino maternal gastrulation neurogenesis |
| 通讯作者 | Yao, JH (reprint author), Fudan Univ, State Key Lab Genet Engn, Inst Genet, Sch Life Sci, Shanghai 200433, Peoples R China.,yaojh@fudan.edu.cn ; jfei@tongji.edu.cn |
| 英文摘要 | Repressor element 1-silencing transcription factor (REST) was recognized as a transcription suppressor regulating nervous system differentiation. However, the role of REST during early development has not been clarified. We cloned the zebrafish homolog of human REST. Real-time polymerase chain reaction results showed that zebrafish REST mRNA was both maternal and zygotic with the higher expression level from blastula to the late segmentation period. Whole-mount in situ hybridization showed that REST was strongly expressed in the blastoderm since dome stage and dynamically expressed mainly in developing brain, especially in the border of the brain subdivisions in early segmentation period. Knockdown of REST using translation blocking morpholino (MO-tra) technique resulted in gastrulation delay or even blockage, and subsequently led to embryo lethality by early segmentation period with deficient neurogenesis. However, splicing blocking morpholino for REST did not show obviously abnormal phenotype until 48 hpf (hours post-fertilization), indicating that maternal REST was an important regulator for gastrulation. Further study revealed that the abnormal development in MO-tra morphants was at least partly due to the dysfunction of protein transportation from the yolk to the blastoderm. Our results showed that REST (especially maternal supplied REST) was required for gastrulation and neurogenesis during zebrafish early embryogenesis. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | EMBRYONIC STEM-CELLS ; TARGET GENES ; FACTOR REST/NRSF ; REST ; EXPRESSION ; REPRESSOR ; SILENCER ; DIFFERENTIATION ; CHROMATIN ; NEURONS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000303333800002 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/599]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wang, XS,Ren, JK,Wang, ZG,et al. NRSF/REST is required for gastrulation and neurogenesis during zebrafish development[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2012,44(5):385-393. |
| APA | Wang, XS,Ren, JK,Wang, ZG,Yao, JH,&Fei, J.(2012).NRSF/REST is required for gastrulation and neurogenesis during zebrafish development.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,44(5),385-393. |
| MLA | Wang, XS,et al."NRSF/REST is required for gastrulation and neurogenesis during zebrafish development".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 44.5(2012):385-393. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。