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Tet3 CXXC Domain and Dioxygenase Activity Cooperatively Regulate Key Genes for Xenopus Eye and Neural Development
文献类型：期刊论文
| 作者 | Xu, YF; Xu, C; Kato, A; Tempel, W; Abreu, JG; Bian, CB; Hu, YG; Hu, D; Zhao, B; Cerovina, T |
| 刊名 | CELL
 |
| 出版日期 | 2012 |
| 卷号 | 151期号:6页码:1200-1213 |
| 通讯作者 | Min, JR (reprint author), Univ Toronto, Struct Genom Consortium, Toronto, ON M5G 1L7, Canada.,jr.min@utoronto.ca ; yoichi.kato@med.fsu.edu ; yujiang_shi@hms.harvard.edu |
| 英文摘要 | Ten-Eleven Translocation (Tet) family of dioxygenases dynamically regulates DNA methylation and has been implicated in cell lineage differentiation and oncogenesis. Yet their functions and mechanisms of action in gene regulation and embryonic development are largely unknown. Here, we report that Xenopus Tet3 plays an essential role in early eye and neural development by directly regulating a set of key developmental genes. Tet3 is an active 5mC hydroxylase regulating the 5mC/5hmC status at target gene promoters. Biochemical and structural studies further demonstrate that the Tet3 CXXC domain is critical for specific Tet3 targeting. Finally, we show that the enzymatic activity and CXXC domain are both crucial for Tet3's biological function. Together, these findings define Tet3 as a transcription regulator and reveal a molecular mechanism by which the 5mC hydroxylase and DNA binding activities of Tet3 cooperate to control target gene expression and embryonic development. |
| 学科主题 | Biochemistry & Molecular Biology; Cell Biology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Cell Biology |
| 关键词[WOS] | EMBRYONIC STEM-CELLS ; DNA METHYLATION ; MAMMALIAN DNA ; SELF-RENEWAL ; 5-HYDROXYMETHYLCYTOSINE ; MOUSE ; 5-METHYLCYTOSINE ; PROTEINS ; GENOME ; 5-CARBOXYLCYTOSINE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000311999900011 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/606]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Xu, YF,Xu, C,Kato, A,et al. Tet3 CXXC Domain and Dioxygenase Activity Cooperatively Regulate Key Genes for Xenopus Eye and Neural Development[J]. CELL,2012,151(6):1200-1213. |
| APA | Xu, YF.,Xu, C.,Kato, A.,Tempel, W.,Abreu, JG.,...&Shi, YG.(2012).Tet3 CXXC Domain and Dioxygenase Activity Cooperatively Regulate Key Genes for Xenopus Eye and Neural Development.CELL,151(6),1200-1213. |
| MLA | Xu, YF,et al."Tet3 CXXC Domain and Dioxygenase Activity Cooperatively Regulate Key Genes for Xenopus Eye and Neural Development".CELL 151.6(2012):1200-1213. |
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