Delicate Analysis of Post-Translational Modifications on Dishevelled 3
文献类型：期刊论文
| 作者 | Wu, CC; Wei, W; Li, C; Li, QR; Sheng, QH; Zeng, R |
| 刊名 | JOURNAL OF PROTEOME RESEARCH
 |
| 出版日期 | 2012 |
| 卷号 | 11期号:7页码:3829-3837 |
| 关键词 | Dvl3 modification SRM dimethylation LTQ-Orbitrap |
| 通讯作者 | Zeng, R (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Key Lab Syst Biol, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.,zr@sibs.ac.cn |
| 英文摘要 | The Wnt/beta-catenin signaling pathway plays a critical role in multiple developmental events during embryogenesis and adult tissue homeostasis. Dishevelled (Dvl) is an important component of Wnt/beta-catenin signaling pathway, although the modification, especially phosphorylation, seems closely related to the activity and stability of Dvl, the overall modification status of Dvl is still poorly understood. In this study, we focused on low abundant Dvl3, one of the three Dvl isoforms. Using affinity purification of different sources of Dvl3, followed by digestion with both trypsin and chymotrypsin, we systematically analyzed the overall modification status of Dvl3 with liquid chromatography coupled LTQ-Orbitrap. Altogether, we confidently identified Dvl3 with more than 50% sequence coverage, including 6 phosphorylation, 4 methylation, and 1 dimethylation sites. Most of the identified modification sites were novel and located in the linker region between different motifs. Subsequently, the validity of modified peptides was confirmed by synthetic modified peptides. Finally, SRM analysis was performed to verify and quantify the successive change of one dimethylation site on Dvl3 in vivo during Wnt signaling. Taken together, our study elucidated the existence of a novel post-translational modification on Dvl3 and provided a delicate experimental procedure for analysis of modification on low abundant proteins. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemical Research Methods |
| 关键词[WOS] | BETA-CATENIN ; ARGININE METHYLATION ; PHOSPHORYLATION ; PATHWAY ; DVL ; PROTEINS ; DISEASE ; SITES ; SILAC |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000306049800027 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/617]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wu, CC,Wei, W,Li, C,et al. Delicate Analysis of Post-Translational Modifications on Dishevelled 3[J]. JOURNAL OF PROTEOME RESEARCH,2012,11(7):3829-3837. |
| APA | Wu, CC,Wei, W,Li, C,Li, QR,Sheng, QH,&Zeng, R.(2012).Delicate Analysis of Post-Translational Modifications on Dishevelled 3.JOURNAL OF PROTEOME RESEARCH,11(7),3829-3837. |
| MLA | Wu, CC,et al."Delicate Analysis of Post-Translational Modifications on Dishevelled 3".JOURNAL OF PROTEOME RESEARCH 11.7(2012):3829-3837. |
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