Misfolded G beta is recruited to cytoplasmic dynein by Nudel for efficient clearance
文献类型：期刊论文
| 作者 | Wan, YH; Yang, ZY; Guo, J; Zhang, QG; Zeng, LY; Song, W; Xiao, Y; Zhu, XL |
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2012 |
| 卷号 | 22期号:7页码:1140-1154 |
| 关键词 | protein degradation dynein-mediated transport G beta protein misfolding signaling |
| 通讯作者 | Zhu, XL (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,xlzhu@sibs.ac.cn |
| 英文摘要 | The G beta gamma heterodimer is an important signal transducer. G beta, however, is prone to misfolding due to its requirement for G gamma and chaperones for proper folding. How cells dispose of misfolded G beta (mfG beta) is not clear. Here, we showed that mfG beta was able to be polyubiquitinated and subsequently degraded by the proteasome. It was sequestered in aggresomes after the inhibition of the proteasome activity with MG132. Sustained activation of G beta gamma signaling further elevated cellular levels of the ubiquitinated G beta. Moreover, Nudel, a regulator of cytoplasmic dynein, the microtubule minus end-directed motor, directly interacted with both the unubiquitinated and ubiquitinated mfG beta. Increasing the levels of both mfG beta and Nudel promoted the association of G beta with both Nudel and dynein, resulting in robust aggresome formation in a dynein-dependent manner. Depletion of Nudel by RNAi reduced the dynein-associated mfG beta, impaired the MG132-induced aggresome formation, and markedly prolonged the half-life of nascent G beta. Therefore, cytosolic mfG beta is recruited to dynein by Nudel and transported to the centrosome for rapid sequestration and degradation. Such a process not only eliminates mfG beta efficiently for the control of protein quality, but may also help to terminate the G beta gamma signaling. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | HETEROTRIMERIC G-PROTEINS ; AGGRESOME FORMATION ; GAMMA-SUBUNITS ; MITOTIC SPINDLE ; TRANSPORT ; COMPLEX ; CELLS ; DEGRADATION ; ASSOCIATION ; CENTROSOME |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000306004200009 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/618]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wan, YH,Yang, ZY,Guo, J,et al. Misfolded G beta is recruited to cytoplasmic dynein by Nudel for efficient clearance[J]. CELL RESEARCH,2012,22(7):1140-1154. |
| APA | Wan, YH.,Yang, ZY.,Guo, J.,Zhang, QG.,Zeng, LY.,...&Zhu, XL.(2012).Misfolded G beta is recruited to cytoplasmic dynein by Nudel for efficient clearance.CELL RESEARCH,22(7),1140-1154. |
| MLA | Wan, YH,et al."Misfolded G beta is recruited to cytoplasmic dynein by Nudel for efficient clearance".CELL RESEARCH 22.7(2012):1140-1154. |
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