AP1 is essential for generation of autophagosomes from the trans-Golgi network
文献类型：期刊论文
| 作者 | Guo, YJ; Chang, CM; Huang, R; Liu, B; Bao, L; Liu, W |
| 刊名 | JOURNAL OF CELL SCIENCE
 |
| 出版日期 | 2012 |
| 卷号 | 125期号:7页码:1706-1715 |
| 关键词 | AP1 LC3 Autophagosome Membrane trafficking Trans-Golgi network |
| 通讯作者 | Liu, W (reprint author), Zhejiang Univ, Sch Med, Dept Biochem & Mol Biol, Program Mol & Cell Biol, Hangzhou 310058, Zhejiang, Peoples R China.,liuwei666@zju.edu.cn |
| 英文摘要 | Despite recent advances in understanding the functions of autophagy in developmental and pathological conditions, the underlying mechanism of where and how autophagosomal structures acquire membrane remains enigmatic. Here, we provide evidence that post-Golgi membrane traffic plays a crucial role in autophagosome formation. Increased secretion of constitutive cargo from the trans-Golgi network (TGN) to the plasma membrane induced the formation of microtubule-associated protein light chain 3 (LC3)-positive structures. At the early phase of autophagy, LC3 associated with and then budded off from a distinct TGN domain without constitutive TGN-to-plasma cargo and TGN-to-endosome proteins. The clathrin adaptor protein AP1 and clathrin localized to starvation-and rapamycin-induced autophagosomes. Dysfunction of the AP1-dependent clathrin coating at the TGN but not at the plasma membrane prevented autophagosome formation. Our results thus suggest an essential role of the TGN in autophagosome biogenesis, providing membrane to autophagosomes through an AP1-dependent pathway. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | ENDOPLASMIC-RETICULUM ; PLASMA-MEMBRANE ; SACCHAROMYCES-CEREVISIAE ; SEC PROTEINS ; LIVING CELLS ; SECRETORY PATHWAY ; VESICLE FORMATION ; CLATHRIN ; ER ; COMPARTMENTS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000303911300011 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/627]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Guo, YJ,Chang, CM,Huang, R,et al. AP1 is essential for generation of autophagosomes from the trans-Golgi network[J]. JOURNAL OF CELL SCIENCE,2012,125(7):1706-1715. |
| APA | Guo, YJ,Chang, CM,Huang, R,Liu, B,Bao, L,&Liu, W.(2012).AP1 is essential for generation of autophagosomes from the trans-Golgi network.JOURNAL OF CELL SCIENCE,125(7),1706-1715. |
| MLA | Guo, YJ,et al."AP1 is essential for generation of autophagosomes from the trans-Golgi network".JOURNAL OF CELL SCIENCE 125.7(2012):1706-1715. |
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