CD44+/CD105+ Human Amniotic Fluid Mesenchymal Stem Cells Survive and Proliferate in the Ovary Long-Term in a Mouse Model of Chemotherapy-Induced Premature Ovarian Failure
文献类型：期刊论文
| 作者 | Liu, T; Huang, YY; Guo, LH; Cheng, WW; Zou, G |
| 刊名 | INTERNATIONAL JOURNAL OF MEDICAL SCIENCES
 |
| 出版日期 | 2012 |
| 卷号 | 9期号:7页码:592-602 |
| 关键词 | Mouse premature ovarian failure model cell transplantation human amniotic fluid cells mesenchymal stem cell like cells long-term survival proliferation |
| 通讯作者 | Liu, T (reprint author), Shanghai Univ Tradit Chinese Med, Longhua Hosp, Shanghai Geriatr Inst Chinese Med, Shanghai 200031, Peoples R China.,liute79@yahoo.com |
| 英文摘要 | Objectives: Stem cell transplantation has been reported to rescue ovarian function in a preclinical mouse model of chemotherapy-induced premature ovarian failure (POF); however, maintaining the survival and self-renewal of transplanted seed cells in ovarian tissues over the long-term remains a troublesome issue. In this study we aimed to determine whether the CD44+/CD105+ human amniotic fluid cell (HuAFCs) subpopulation represent potential seed cells for stem cell transplantation treatments in POF. Materials and methods: The CD44+/CD105+ subpopulation were isolated from HuAFCs, cultured in vitro, and injected into a cyclophosphamide-induced mouse model of POF. Results: Under continuous subculture in vitro, CD44+/CD105+ cells proliferated rapidly and expressed high levels of the proliferative markers Ki67 and survivin, as well as high levels of a number of mesenchymal stem cell biomarkers. Moreover, when red fluorescence protein (RFP)-transduced CD44+/CD105+ HuAFCs were transplanted into the ovaries of POF mice, the cells could be detected by fluorescence microscopy up to three weeks after injection. Furthermore, the BrdUrd incorporation assay and immunofluorescent staining demonstrated that CD44+/CD105+ HuAFCs underwent normal cycles of cell proliferation and self-renewal in the ovarian tissues of POF mice over the long-term. Conclusions: The mesenchymal stem cell properties and long-term in vivo survival of CD44+/CD105+ HuAFCs make them ideal seed cells for stem cell transplantation to treat POF. |
| 学科主题 | General & Internal Medicine |
| 类目[WOS] | Medicine, General & Internal |
| 关键词[WOS] | RAT ; TRANSPLANTATION ; LINES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000310128500010 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/628]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Liu, T,Huang, YY,Guo, LH,et al. CD44+/CD105+ Human Amniotic Fluid Mesenchymal Stem Cells Survive and Proliferate in the Ovary Long-Term in a Mouse Model of Chemotherapy-Induced Premature Ovarian Failure[J]. INTERNATIONAL JOURNAL OF MEDICAL SCIENCES,2012,9(7):592-602. |
| APA | Liu, T,Huang, YY,Guo, LH,Cheng, WW,&Zou, G.(2012).CD44+/CD105+ Human Amniotic Fluid Mesenchymal Stem Cells Survive and Proliferate in the Ovary Long-Term in a Mouse Model of Chemotherapy-Induced Premature Ovarian Failure.INTERNATIONAL JOURNAL OF MEDICAL SCIENCES,9(7),592-602. |
| MLA | Liu, T,et al."CD44+/CD105+ Human Amniotic Fluid Mesenchymal Stem Cells Survive and Proliferate in the Ovary Long-Term in a Mouse Model of Chemotherapy-Induced Premature Ovarian Failure".INTERNATIONAL JOURNAL OF MEDICAL SCIENCES 9.7(2012):592-602. |
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