Nudel is crucial for the WAVE complex assembly in vivo by selectively promoting subcomplex stability and formation through direct interactions
文献类型：期刊论文
| 作者 | Wu, S; Ma, L; Wu, YB; Zeng, R; Zhu, XL |
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2012 |
| 卷号 | 22期号:8页码:1270-1284 |
| 关键词 | Nudel Sra1 HSPC300 the WAVE regulatory complex assembly stabilization |
| 通讯作者 | Zhu, XL (reprint author), Chinese Acad Sci, State Key Lab Cell Biol, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,xlzhu@sibs.ac.cn |
| 英文摘要 | The WAVE regulatory complex (WRC), consisting of WAVE, Sra, Nap, Abi, and HSPC300, activates the Arp2/3 complex to control branched actin polymerization in response to Rac activation. How the WRC is assembled in vivo is not clear. Here we show that Nudel, a protein critical for lamellipodia formation, dramatically stabilized the Sra1-Nap1-Abi1 complex against degradation in cells through a dynamic binding to Sra1, whereas its physical interaction with HSPC300 protected free HSPC300 from the proteasome-mediated degradation and stimulated the HSPC300-WAVE2 complex formation. By contrast, Nudel showed little or no interactions with the Sra1-Nap1-Abi1-WAVE2 and the Sra1-Nap1-Abi1-HSPC300 complexes as well as the mature WRC. Depletion of Nudel by RNAi led to general subunit degradation and markedly attenuated the levels of mature WRC. It also abolished the WRC-dependent actin polymerization in vitro and the Rac1-induced lamellipodial actin network formation during cell spreading. Therefore, Nudel is important for the early steps of the WRC assembly in vivo by antagonizing the instability of certain WRC subunits and subcomplexes. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | CYTOPLASMIC DYNEIN ; ACTIN ; CELLS ; CDC42 ; BINDING ; LIS1 ; CYTOSKELETON ; DEGRADATION ; ASSOCIATION ; ACTIVATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000307119500011 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/630]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wu, S,Ma, L,Wu, YB,et al. Nudel is crucial for the WAVE complex assembly in vivo by selectively promoting subcomplex stability and formation through direct interactions[J]. CELL RESEARCH,2012,22(8):1270-1284. |
| APA | Wu, S,Ma, L,Wu, YB,Zeng, R,&Zhu, XL.(2012).Nudel is crucial for the WAVE complex assembly in vivo by selectively promoting subcomplex stability and formation through direct interactions.CELL RESEARCH,22(8),1270-1284. |
| MLA | Wu, S,et al."Nudel is crucial for the WAVE complex assembly in vivo by selectively promoting subcomplex stability and formation through direct interactions".CELL RESEARCH 22.8(2012):1270-1284. |
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