Cloning and primary characterizations of rLcn9, a new member of epididymal lipocalins in rat
文献类型：期刊论文
| 作者 | Li, XQ; Zhan, XN; Liu, SG; Hu, SG; Zhu, CF; Hall, SH; French, FS; Liu, Q; Zhang, YL |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2012 |
| 卷号 | 44期号:10页码:876-885 |
| 关键词 | epididymis lipocalin Lcn9 gene |
| 通讯作者 | Liu, Q (reprint author), Chinese Acad Sci, Inst Biochem & Cell Biol, Shanghai Key Lab Mol Androl, State Key Lab Mol Biol,Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China.,qliu@sibs.ac.cn ; ylzhang@sibs.ac.cn |
| 英文摘要 | Lipocalins are a structurally conserved and diversely functional family of proteins that are of potential importance in epididymis functions. The rat Lcn9 gene was cloned by in silico methods and genome walking based on homology to the rhesus monkey epididymal ESC513 and its polyclonal antisera were prepared. The rat Lcn9 gene is located on chromosome 3p13 spanning 7 exons, contains 2.3 kb and encodes 179 amino acids with a 17-amino acid signal peptide. Northern blot, western blot, and immunohistochemical staining analysis revealed that rat Lcn9 was a novel epididymis-specific gene, expressed selectively in the proximal caput region, influenced by luminal fluid testicular factors. Moreover, Lcn9 protein was modified by N-glycosylation and bound on the postacrosomal domain of caput sperm. In conclusion, the rat Lcn9 exhibited tissue-, region-, and temporal-specific expression patterns and its expression was regulated by luminal testicular factors. Its potential roles in sperm maturation are discussed. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | ACID-BINDING PROTEIN ; PROSTAGLANDIN-D SYNTHASE ; GENE-EXPRESSION ; SECRETORY PROTEINS ; SPERM MATURATION ; BETA-DEFENSIN ; LOCALIZATION ; FAMILY ; MOUSE ; TESTIS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000310370500009 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/649]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Li, XQ,Zhan, XN,Liu, SG,et al. Cloning and primary characterizations of rLcn9, a new member of epididymal lipocalins in rat[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2012,44(10):876-885. |
| APA | Li, XQ.,Zhan, XN.,Liu, SG.,Hu, SG.,Zhu, CF.,...&Zhang, YL.(2012).Cloning and primary characterizations of rLcn9, a new member of epididymal lipocalins in rat.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,44(10),876-885. |
| MLA | Li, XQ,et al."Cloning and primary characterizations of rLcn9, a new member of epididymal lipocalins in rat".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 44.10(2012):876-885. |
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