Structural insights into SUN-KASH complexes across the nuclear envelope
文献类型：期刊论文
| 作者 | Wang, WJ; Shi, ZB; Jiao, S; Chen, CC; Wang, HZ; Liu, GG; Wang, Q; Zhao, Y; Greene, MI; Zhou, ZC |
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2012 |
| 卷号 | 22期号:10页码:1440-1452 |
| 关键词 | crystal structure SUN2 KASH LINC complexes mechanical force transduction nuclear envelope |
| 通讯作者 | Zhou, ZC (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Cell Biol, Shanghai 200031, Peoples R China.,zczhou@sibs.ac.cn |
| 英文摘要 | Linker of the nucleoskeleton and the cytoskeleton (LINC) complexes are composed of SUN and KASH domain-containing proteins and bridge the inner and outer membranes of the nuclear envelope. LINC complexes play critical roles in nuclear positioning, cell polarization and cellular stiffness. Previously, we reported the homotrimeric structure of human SUN2. We have now determined the crystal structure of the human SUN2-KASH complex. In the complex structure, the SUN domain homotrimer binds to three independent "hook"-like KASH peptides. The overall conformation of the SUN domain in the complex closely resembles the SUN domain in its apo state. A major conformational change involves the AA'-loop of KASH-bound SUN domain, which rearranges to form a mini beta-sheet that interacts with the KASH peptide. The PPPT motif of the KASH domain fits tightly into a hydrophobic pocket on the homotrimeric interface of the SUN domain, which we termed the BI-pocket. Moreover, two adjacent protomers of the SUN domain homotrimer sandwich the KASH domain by hydrophobic interaction and hydrogen bonding. Mutations of these binding sites disrupt or reduce the association between the SUN and KASH domains in vitro. In addition, transfection of wild-type, but not mutant, SUN2 promotes cell migration in Ovcar-3 cells. These results provide a structural model of the LINC complex, which is essential for additional study of the physical and functional coupling between the cytoplasm and the nucleoplasm. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | CAENORHABDITIS-ELEGANS ; MEMBRANE PROTEIN ; LINC COMPLEXES ; MIGRATION ; LOCALIZATION ; MATEFIN/SUN-1 ; LAMINOPATHIES ; REQUIREMENTS ; CHROMOSOMES ; TELOMERES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000309518300009 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/650]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wang, WJ,Shi, ZB,Jiao, S,et al. Structural insights into SUN-KASH complexes across the nuclear envelope[J]. CELL RESEARCH,2012,22(10):1440-1452. |
| APA | Wang, WJ.,Shi, ZB.,Jiao, S.,Chen, CC.,Wang, HZ.,...&Zhou, ZC.(2012).Structural insights into SUN-KASH complexes across the nuclear envelope.CELL RESEARCH,22(10),1440-1452. |
| MLA | Wang, WJ,et al."Structural insights into SUN-KASH complexes across the nuclear envelope".CELL RESEARCH 22.10(2012):1440-1452. |
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