Haploid embryonic stem cells: an ideal tool for mammalian genetic analyses
文献类型：期刊论文
| 作者 | Shi, LY; Yang, H; Li, JS |
| 刊名 | PROTEIN & CELL
 |
| 出版日期 | 2012 |
| 卷号 | 3期号:11页码:806-810 |
| 关键词 | haploid embryonic stem cells parthenogenetic embryos androgenetic embryos genetic screening diploid genomic imprinting |
| 通讯作者 | Li, JS (reprint author), Chinese Acad Sci, Inst Biochem & Cell Biol, Grp Epigenet Reprogramming, State Key Lab Cell Biol, Shanghai 200031, Peoples R China.,jsli@sibs.ac.cn |
| 英文摘要 | Identification of the function of all genes in the mammalian genome is critical in understanding basic mechanisms of biology. However, the diploidy of mammalian somatic cells has greatly hindered efforts to elucidate the gene function in numerous biological processes by mutagenesis-based genetic approaches. Recently, mouse haploid embryonic stem (haES) cells have been successfully isolated from parthenogenetic and androgenetic embryos, providing an ideal tool for genetic analyses. In these studies, mouse haES cells have already shown that they could be used in cell-based forward or reverse genetic screenings and in generating gene-targeting via homologous recombination. In particular, haES cells from androgenetic embryos can be employed as novel, renewable form of fertilization agent for yielding live-born mice via injection into oocytes, thus showing the possibility that genetic analysis can be extended from cellular level to organism level. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | 33342 INDUCES APOPTOSIS ; MOUSE EMBRYOS ; IN-VITRO ; GENERATION ; ESTABLISHMENT ; EXPRESSION ; DERIVATION ; SCREENS ; CULTURE ; ART |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000310970600002 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/656]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Shi, LY,Yang, H,Li, JS. Haploid embryonic stem cells: an ideal tool for mammalian genetic analyses[J]. PROTEIN & CELL,2012,3(11):806-810. |
| APA | Shi, LY,Yang, H,&Li, JS.(2012).Haploid embryonic stem cells: an ideal tool for mammalian genetic analyses.PROTEIN & CELL,3(11),806-810. |
| MLA | Shi, LY,et al."Haploid embryonic stem cells: an ideal tool for mammalian genetic analyses".PROTEIN & CELL 3.11(2012):806-810. |
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